Anaerobic biodegradation of unsaturated, saturated, aromatic and

Liquid purification or separation – Processes – Treatment by living organism

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

210903, 210909, C02F 328

Patent

active

059421177

ABSTRACT:
An apparatus and method for anaerobic biodegradation, bioremediation or bioprocessing of hydrocarbons dissolved in an aqueous matrix, such as wastewater, groundwater, or slurry. Dissolved alkanes (saturated hydrocarbons), alkenes (unsaturated hydrocarbons), aromatic hydrocarbons and/or halogenated hydrocarbons are metabolized or cometabolized. In one form, the invention involves introducing an aqueous stream comprising at least one dissolved aromatic hydrocarbon (such as benzene, toluene, ethylbenzene, o-xylene, m-xylene, p-xylene, phenol, o-cresol, m-cresol, or (NO.sub.3.sup.-), nitrite (NO.sub.2.sup.-), nitric oxide (NO) and nitrous oxide (N.sub.2 O)!to a reactor, and operating said reactor under conditions that support denitrification of the aromatic hydrocarbon. Alternatively, the aqueous stream may comprise at least one alkane (such as ethane) and/or at least one alkene (such as ethene or ethylene) and biodegradation of these compounds is accomplished. In a preferred form, the aqueous stream also comprises at least one dissolved halogenated hydrocarbon (such as tetrachloroethylene, trichloroethylene, or 1,1,1-trichloroethane) and dehalogenation of the halogenated hydrocarbon is accomplished. The reactor may be a continuous stirred tank reactor, a batch (or sequencing batch) reactor, a plug-flow reactor, a fixed-film reactor, or a pore space in an underground aquifer in situ. The reactor is operated in such a way that molecular oxygen is excluded from the space or zone in which the biodegradation is occurring and the other requirements of denitrifying bacteria are met. In some implementations, kinetic control (control of mean cell residence time) is used to enrich a denitrifying culture in the reactor.

REFERENCES:
patent: 4352886 (1982-10-01), Pillis et al.
patent: 5024949 (1991-06-01), Hegeman et al.
Haner, A., Hohener, P., & Zeyer, J. (1995). Degradation of p-xylene by a denitrifying enrichment culture. Applied and Environmental Microbiology, 61, 3185.
Lovley, D.R., Woodward, J.C. & Chapelle, F.H. (1996). Rapid anaerobic benzene oxidation with a variety of chelated Fe(III) forms. Appl. Environ. Microbiol, 62, 288-291.
Lovley, D.R., Coates, J.D., Woodward, J.C. & Phillips, E.J.P. (1995). Benzene oxidation coupled to sulfate reduction. Appl. Environ. Microbiol, 61, 953-958.
Grady, C.P.L., Jr. (1989). Biological detoxification of hazardous wastes: What do we know? What should we know? In Y.C. Wu (Ed.), Proceedings of the International Conference on Physiochemical and Biological Detoxificationof Hazardous Wastes, (pp. 3-16). Lancaster, Pennsylvania: Technomic.
Bakker, G. (1977). Anaerobic degradation of aromatic compounds in the presence of nitrate. FEMS Microbiol. Lett., 1, 103-108.
Molin, G. & Nilssan, I. (1985). Degradation of phenol by Pseudomonas putida ATCC 11172 in continuous culture at different ratios of biofilm surface to culture volume. Appl. Environ. Microbiol., 50, 946-950.
Bossert, I.D., Rivera, M.D., & Young, L.Y. (1986). p-Cresol biodegradation under denitrifying conditions: Isolation of a bacterial coculture. FEMS Microbiology Ecology, 38, 313-319.
Bossert, I.D. & Young, L.Y. (1986). Anaerobic oxidation of p-cresol by a denitrifying bacterium. Appl. Environ. Microbiol., 52, 1117-1122.
Tschech, A. & Fuchs, G. (1987). Anaerobic degradation of phenol by pure cultures of newly isolated denitrifying pseudomonads. Arch. Microbiol., 148, 213-217.
Hu, L.Z. & Shieh, W.K. (1987). Anoxic biofilm degradation of monocyclic aromatic compounds. Biotech. Bioeng., 30, 1077-1083.
Major, D.W., Mayfield, C.I., & Barker, J.F. (1988). Biotransformation of benzene by denitrification in aquifer sand. Ground Water, 26, 8-14.
Kuhn, E.P., Zeyer, J., Eicher, P, & Schwarzenbach, R.P. (1988). Anaerobic degradation of alkylated benzenes in denitrifying laboratory aquifer columns. Appl. Environ. Microbiol., 54, 490-496.
Zache & Rehm. (1989). Degradation of phenol by a coimmobilized entrapped mixed culture. Appl. Microbial Biotech., 30, 426-432.
Haggblom, M.M., Rivera, M.D., Bosser, I.D., Rogers, J.E., & Young, L.Y. (1990). Anaerobic biodegradation of para-cresol under three reducing conditions. Microbial Ecology, 20, 141-150.
Evans, P.J., Mang, D.T., Kim, K.S., & Young, L.Y. (1991a). Anaerobic degradation of toluene by a denitrifying bacterium. Appl. Environ. Microbiol., 57, 1139-1145.
Evans, P.J., Mang, D.T., & Young, L.Y. (1991b). Degradation of toluene and m-xylene and transformation of o-xylene by denitrifying enrichment cultures. Appl. Environ. Microbiol., 57, 450-454.
Hutchins, S.R. (1991). Biodegradation of monoaromatic hydrocarbons by aquifer microorganisms using oxygen, nitrate, or nitrous oxide as the terminal electron acceptor. Appl. Environ. Microbiol., Aug., 2403-2407.
Evans, P.J., Ling, W., Goldschmidt, B., Ritter, E.R., & Young, L.Y. (1992). Metabolites formed during anaerobic transformation of toluene and O-xylene and their proposed relationship to the initial steps of toluene mineralization. Appl. Environ. Microbiol., Feb., 496-501.
Khoury, N., Dott, W., & Kampfer, P. (1992). Anaerobic degradation of p-cresol in batch and continuous cultures by a denitrifying bacterial consortium. Appl. Microbiol. Biotech., 37, 529-531.
Khoury, N., Dott, W., & Kampfer, P. (1992). Anaerobic degradation of phenol in batch and continuous cultures by a denitrifying bacterial consortium. Appl. Microbiol. Biotech., 37, 524-528.
Coschigano, P.W., Haggblom, M.M., & Young, L.Y. (1994). Metabolism of both 4-Chlorobenzoate and Toluene under denitrifying conditions by a constructed bacterial strain. Appl. Environ. Microbiol., 60, 989-995.
Seyfried, B., Glod, G., Schocher, R., Tschech, A., & Zeyer, J. (1994). Initial reactions in the anaerobic oxidation of toluene and m-xylene by denitrifying bacteria. Appl. Environ. Microbiol., 60, 4047-4052.
Fries, M.R., Zhou, J., Chee-Sanford, J., & Tiedje, J.M. (1994). Isolation, characterization, and distribution of denitrifying toluene degraders from a veriety of habitats. Appl. Environ. Microbiol., 60, 2802-2810.
Bouwer, E.J. & McCarty, P.L. (1983). Transformations of 1- and 2-carbon halogenated aliphatic organic compounds under methanogenic conditions. Appl. Environ. Microbiol., 45, 1286-1294.
Egli, C., Tschan, T., Scholtz, R., Cook, A.M., & Leisinger, T. (1988). Transformation of tetrachloromethane to dichloromethane and carbon dioxide by Acetobacterium woodii. Appl. Environ. Microbiol., 54, 2819-2824.
Criddle, C.S., DeWitt, J.T., Grbi-Gali, D., & McCarty, P.L. (1990). Transformation of carbon tetrachloride by Pseudomonas sp. Strain KC under denitrificaiton conditions. Appl. Environ. Microbiol., 56, 3240-3246.
Petersen, J.N., Skeen, R.S., Amos, K.M., & Hooker, B.S. (1994). Biological destruction of CC14: I. Experiemental design and date. Biotech. Bioeng., 43, 521-528.
Hooker, B.S., Skeen, R.S., & Petersen, J.N. (1994). Biological destruction of Cc14: II. Kinetic modeling. Biotech. Bioeng., 44, 211-218.
Skeen, R.S., Truex, M.J., Persen, J.N., & Hill, J.S. (1994). A batch reactor for monitoring process dynamics during biodegradation of volatile organics. Environmental Progress, 13, 174-176.
Grady, C.P.L. Jr. & Lim, G.C. (1980). Biological Wastewater Treatment. New York: Marcel Dekker.
Aeckersberg, F., Bak, F., & Widdel, F. (1991). Anaerobic oxidation of saturated hydrocarbons to CO2 by a new type of sulfate-reducing bacterium. Arch. Microbiol., 156, 5-14.
Rabus, R., Nordhaus, R., Ludwig, W., & Widdel, F. (1993). Complete oxidation of toluene under strictly anoxic conditions by a new sulfate-reducing bacterium. Appl. Environ. Microbiol., 59, 1444-1451.
Edwards, E.A., Wills, L.E., Reinhard, M., & Grbic-Galic, D. (1992). Anaerobic degradation of toluene and xylene by aquifer microorganisms under sulfate-reducing conditions. Appl. Environ. Microbiol., 58, 794-800.
Beller, H.R., Grbic-Galic, D., & Reinhard, M. (1992). Microbial degradation of toluene under sulfate-reducing conditions and the influence of iron on the process. Appl. Environ. Microbiol., 58, 786-793.
Tandol, V., DiStefano, T.D., Bowser, P.A., Gossett, J.M., & Zinder, S.H. (1994). Reductive dehalogenati

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Anaerobic biodegradation of unsaturated, saturated, aromatic and does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Anaerobic biodegradation of unsaturated, saturated, aromatic and, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Anaerobic biodegradation of unsaturated, saturated, aromatic and will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-463523

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.