Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including perfusion; composition therefor
Patent
1993-06-18
1995-04-18
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
Including perfusion; composition therefor
4352402, 514 3, 514 23, 514 46, 514561, A01N 102, C12N 500, A61K 3726, A61K 3170, A61K 31195
Patent
active
054077931
ABSTRACT:
A process for preserving a patient's organ for transplantation and cardioplegia during cardiac surgery is disclosed which includes harvesting the patient's organ for transplantation or arresting the heart during cardiac surgery, perfusing the organ or heart with an aqueous solution, and removing at least a substantial portion of the aqueous solution from the organ or heart to effect the removal of waste products from the organ before transplantation or restarting the heart. The aqueous solutions of these inventions promote anaerobic glycolysis, remove waste products from the organ and maintain the energy production capacity of the organ and are preferably comprised of about 50 to 150 millimoles of histidine per liter of the solution, at least one energy providing material for maintaining the energy production capacity of the organ, about 60 to 90 millimoles of Na.sup.+ per liter of the solution, about 10 to 25 millimoles of K.sup.+ per liter of the solution, and about 3 to 8 millimoles of adenosine per liter of the solution for the transplantation solution and 0.001 to 1 millimoles of adenosine per liter of the solution for cardioplegia. Sodium entry into and depolarization of the cells is resisted by the extracellular to intracellular sodium gradient and use of adenosine. The aqueous solutions of this invention have a pH of about 6.8 to 8.0 where intracellular pH is maintained by the buffering action of the histidine and the solution is preferably used at moderate hypothermia for up to 24 hours.
REFERENCES:
patent: 4415556 (1983-11-01), Bretschneider
patent: 4663289 (1987-05-01), Veech
patent: 4798824 (1989-01-01), Belzer et al.
patent: 4873230 (1989-10-01), Belzer et al.
patent: 4920044 (1990-04-01), Bretan, Jr.
patent: 4981691 (1991-01-01), Osterholm et al.
patent: 5082831 (1992-01-01), Leaf et al.
patent: 5145771 (1992-09-01), Lemasters et al.
del Nido et al., "The Role of Cardioplegic Solution Buffering in Myocardial rotection", The Journal of Thoracic and Cardiovascular Surgery, vol. 89, No. 5, pp. 689-699, May (1985).
Jimenez et al., "Effects of Low Extracellular Calcium on Cytosolic Calcium and Ischemic Contracture", Journal of Surgical Research, vol. 49, pp. 252-255 (1990).
Qayumi et al., "Preservation Techniques for Heart Transplantation: Comparison of Hypothermic Storage and Hypothermic Perfusion", J. Heart Lung Transplant, Jul.-Aug. 1991, vol. 10, No. 4, pp. 518-526.
Cao-Danh Hung
Del Nido Pedro J.
Ohkado Akihiko
Sommers K. Eric
Appleman Jolene W.
Dadio Susan M.
Robinson Douglas W.
Silverman Arnold B.
University of Pittsburgh of the Commonwealth System of Higher Ed
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