Amylase variants

Details Amylase variants Amylase variants

1996-10-10

1998-05-19

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352523, 4353201, 435202, 435203, 435204, 536 232, 536 237, 510226, C12P 2106, C12N 120, C12N 1500, C07H 2104

Patent

active

057534608

ABSTRACT:
A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.

REFERENCES:
patent: 5093257 (1992-03-01), Gray
Suzuki et al., J.of Biol. Chem., vol. 264, No. 32, pp. 18933-18938 (1989).
10th Enzyme Engineering Conference, Programss and Abstracts -- Diderichsen, et al., Chimeric Alpha-amylases, Kashi Kojima Japan, Sep. 24-29, 1989, Poster No. I-7.
Suzuki et al. J. Biol. Chem. (1989), 264(32):18933-18938, Nov. 15, 1989.

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