Amylase-negative, asporogenous mutant of Bacillus subtilis usefu

Details Amylase-negative, asporogenous mutant of Bacillus subtilis usefu Amylase-negative, asporogenous mutant of Bacillus subtilis usefu

1982-04-21

1984-08-14

Goldberg, Jerome D.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351723, 435839, 935 56, C12N 120, C12N 1500, C12R 1125

Patent

active

044657730

ABSTRACT:
A biologically pure amylase-negative, asporogenous mutant B. subtilis EE101 (ATCC 39,096) is provided. This host, which contains no amylase-coding gene, is particularly useful as a host in a host-vector system for recombinant DNA work directed toward the production of improved strains of amylase-producing microorganisms.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4302544 (1981-11-01), Young et al.
Ellis, D. M. and Dean, D. H., Recombinant DNA Technical Bulletin, vol. 4, No. 1, Mar. 1981.
Burke, W. F. and Le, H. T., Recombinant DNA Technical Bulletin, vol. 3, No. 1, Dec. 1980.
Goldthwaite, Dubnau and Smith, Proc. Natl. Acad. Sci., U.S.A., 65, 96-103 (1970).

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