Textiles: fiber preparation
Patent
1977-07-28
1978-07-25
Jones, Raymond N.
Textiles: fiber preparation
G01N 3114
Patent
active
041027477
ABSTRACT:
A method for the determination of amylase, based on the cleavage of colorimetric, ultraviolet absorbing or fluorometric substances, commonly referred to as chromogenic substances as p-nitrophenol derivatives of oligosaccharides of chain length 4-10 glucose units. Oligosaccharides of this chain length are resistant to cleavage by .alpha. and/or .beta.-glucosidase and contain endo-.alpha.-1,4 linkages which are required for amylase activity. Cleavage of the .alpha.-1,4 bonds by amylase produces smaller fragments which are acted upon by .alpha.-glucosidase and/or .beta.-glucosidase to liberate a chromophore. The rate of appearance of the chromophore is proportional to amylase activity and lends itself to either rate or endpoint determinations. The qualitative or quantitative measurement of amylase concentrations in a sample is useful in medical diagnosis.
REFERENCES:
patent: 3679661 (1972-07-01), Babson
patent: 3694318 (1972-09-01), Klein
Bergmeyer "Methods of Enzymatic Analysis", Academic Press, Inc., New York (1972) pp. 885-898.
Janben et al., Nature vol. 182 (1958) pp. 525 & 526.
Driscoll Richard Cornelius
Gargiulo Robert James
Giegel Joseph L.
American Hospital Supply Corporation
Azulay Y. Judd
Fan C. A.
Jones Raymond N.
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