Amylase-containing detergent compositions

Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – For cleaning a specific substrate or removing a specific...

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Details

510309, 510320, 510372, 510374, 510375, 510392, 510530, 435203, C11D 3386, C11D 330, C11D 336

Patent

active

057835462

DESCRIPTION:

BRIEF SUMMARY
TECHNICAL FIELD

The present invention relates to detergent compositions containing specific amylases.


BACKGROUND OF THE INVENTION

The use of amylases in detergent compositions is well known in the art.
The practical use of amylases in detergents has however been limited so far to bacterial amylases such as Termamyl.RTM., BAN.RTM. sold by Novo Nordisk, Maxamyl.RTM. and ex Gist-Brocades, Rapidase.RTM..
On the other hand, certain fungal amylases are being sold by Novo Nordisk under the trade name Fungamyl.RTM., for use in the starch industry, brewing industry, alcohol industry, and baking.
In the starch industry, Fungamyl.RTM. is used for production of high maltose syrups, or high conversion syrups. In the brewing industry, Fungamyl.RTM. is added during fermentation in order to increase fermentability of the wort. In the alcohol industry, Fungamyl.RTM. may be used for liquefaction of starch in a distillery mash. In baking, Fungamyl.RTM. is used for supplementation of wheat flour low in alpha-amylase.
Fungamyl.RTM. is however not used nor suggested for use, in detergent applications; furthermore, the trade brochure from Novo Nordisk, indicates that alkaline pHs have a detrimental effect on Fungamyl.RTM. activity.
It has now been surprisingly found that Fungamyl.RTM. can provide substantial cleaning benefits, especially in laundry context as regards whiteness maintenance and cleaning of body soils, these benefits being seen as well at alkaline pHs.
It has also been found that said Fungamyl.RTM. enzymes exhibit CMCase activity, contrary to the currently used amylase described above.
It is accordingly the object of the present invention to provide detergent compositions containing an amylase enzyme which showing CMCase activity and/or is an amylase showing a positive immunological cross reaction with the antibody of the Fungamyl.RTM. amylase, or an amylase produced by a host organism in which the gene encoding the Fungamyl.RTM. has been cloned.


SUMMARY OF THE INVENTION

A detergent composition comprising an amylase enzyme which shows CMCase activity and/or is an amylase showing a positive immunological cross reaction with the antibody of the Fungamyl.RTM. amylase, or an amylase produced by a host organism in which the gene encoding the Fungamyl.RTM. has been cloned.


DETAILED DESCRIPTION OF THE INVENTION



The Amylase

The compositions herein comprise as an essential ingredient a specific amylase, which shows CMCase and/or is an amylase showing a positive immunological cross reaction with the antibody of the Fungamyl.RTM. amylase, or an amylase produced by a host organism in which the gene encoding the Fungamyl.RTM. has been cloned. Fungamyl.RTM. is described by Novo Nordisk trade brochure dated February 1993, as being a 1,4- -D-glucan glucano-hydrolase, obtainable from a selected stain of Asperillys oryzae.
The CMCase activity of the amylase herein is defined and as follows: NCOH and 50C, in the presence of amylase (0.05 g of an amylase solution at 800 FAU/g). The amylase is considered to have CMCase activity if after 1 minute incubation, at least 0.1, more preferably 0.5, most preferable 1 ppm or more glucose is formed as detected by ion chrmoatography/pulsed amperometric detection (IC/PAD). 100 ml of the CMC (1000 g/l) solution. column. H20/100 mM NaOH/500 mM Na Acetate eluents were used.


______________________________________ E1 + 0.05 480 E2 + 0.06 300 E3 - 0.61 240 ______________________________________
The amylases of the present invention are preferably fungal and an example thereof is Fungamyl itself.
The Fungamyl.RTM. amylase herein should be used in a substantially pure form. By substantially pure form is meant, that the specific fungal alpha amylase of the invention should not be present in the form of an impurity included in another enzyme, separately added to the present composition to provided a different function.
However, it is understood that the substantially pure Fungamyl.RTM. can be used in conjunction with other enzymes.
The level of the fungal amylase in the composit

REFERENCES:
patent: 3416997 (1968-12-01), Barton
patent: 4011169 (1977-03-01), Diehl et al.
patent: 4142999 (1979-03-01), Bloching et al.
patent: 4377602 (1983-03-01), Conrad
patent: 4381247 (1983-04-01), Nakawaga et al.
patent: 4551177 (1985-11-01), Trubiano et al.
patent: 5312557 (1994-05-01), Onda et al.
patent: 5332528 (1994-07-01), Pan et al.
patent: 5356800 (1994-10-01), Jacquess et al.
patent: 5474701 (1995-12-01), Jacquess et al.
patent: 5507952 (1996-04-01), Jacquess et al.

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