Amylase assay

Details Amylase assay Amylase assay

1978-02-27

1980-11-11

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435810, C12Q 140

Patent

active

042334039

ABSTRACT:
Disclosed herein are a method and a reagent test kit, both using an improved substrate to measure the amylase content of a sample. The substrate used is a glycoside consisting of a defined polysaccharide glycosyl residue and a substituted aromatic radical attached to the terminal unit of the glycoside. When detached from the polysaccharide, the aglycone exhibits a different spectral absorbance than the substrate.

REFERENCES:
patent: 3476515 (1969-11-01), Johnson et al.
patent: 3879263 (1975-04-01), Adams
patent: 4000042 (1976-12-01), Adams
patent: 4009079 (1977-02-01), Tsujino et al.
patent: 4102749 (1978-07-01), Driscoll et al.
Hamley G. G. The Condensed Chemical Dictionary, 8th ed. Van Nostrand Reinhold Co. N. Y., 1971 (pp. 346, 419 and 539).
Jansen et al. .alpha.-(p-Nitrophenyl) Maltocide as a Substrate for the Assay of Amylase. Nature, vol. 182, 1958 (pp. 525-526).
Driscoll, et al., A New Method for the Determination of Amylase 173rd ACS Meeting, New Orleans, Louisiana 3/1977, Abstract No. 117.

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