Amplified hybridization assay

Details Amplified hybridization assay Amplified hybridization assay

1992-10-20

1995-06-13

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, 935 77, 935 78, C12Q 168, C07H 2104, C12N 1500

Patent

active

054241880

ABSTRACT:
A kit for an amplified hybridization assay is described in which a family of signal-generating secondary probes bind to a primary probe that hybridizes to the target sequence of interest. Thus, an enormously amplified signal is generated by the hybridization event. The assay can be used for a variety of laboratory and clinical purposes and is automatable. A hybridization assay kit is also described. The kit is used for the detection of a target nucleotide sequence. One embodiment of the kit includes a plurality of secondary probes, each secondary probe capable of binding to a distinct binding site of the primary probe.

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