Amplified expression vector

Details Amplified expression vector Amplified expression vector

1983-04-04

1989-04-11

Marinell, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 68, 4351721, 4351723, 435243, 435320, 536 27, 935 29, 935 41, 935 72, 935 73, C12N 120, C12N 1500, C12N 100, C12P 2100, C07H 2104

Patent

active

048206423

ABSTRACT:
A DNA expression vector which contains the trp promotor is described. The expression vector provides for the overproduction of .beta.-lactamase. Insertion of a gene or cDNA into the .beta.-lactamase gene of the expression vector results in the over-production of a fusion protein comprising a part of the .beta.-lactamase as the N-terminal end and the protein coded for by the inserted DNA as the C-terminal end. Using the expression vector described herein, it is possible to obtain large amounts of the fusion protein. A fusion protein containing the surface antigen of Hepatitis B virus and a vaccine containing this fusion protein are also described.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4411994 (1983-10-01), Gilbert et al.
patent: 4415491 (1979-11-01), Vyas
patent: 4418149 (1983-11-01), Ptashne et al.
patent: 4428941 (1984-01-01), Galibert et al.
patent: 4431739 (1984-02-01), Riggs
Fritsch et al., "Clonage du Genome du Virus de l'Hepatite B Dans Escherichia coil", C. R. Acad. Sc. Paris, Series D, 287: 1453. (1978).
Galibert et al., "Nucleotide Sequence of the Hepatits B Virus Genome (Subtype ayw) Cloned in E. coli", Nature 281: 646 (1979).
Burrell et al., "Expression in Escherichia coli of Hepatitis B Virus DNA Sequences Cloned in Plasmid pBR322", Nature 279: 43 (1979).
Pasek et al., "Hepatits B Virus Genes and Their Expression in E. coli", Nature 282: 575 (1979).
Stauffer et al., "In Vivo Cloning of DNA Regions Carrying Mutations Linked to Selectable Genes: Applications to Mutations in the Regulatory Region of the Escherichia coli Tryptophan Operon", Plasmid 2: 498 (1979).
Hallewell et al., "Plasmid Vectors Containing Tryptophan Operon Promotor Suitable for Efficient Regulated Expression of Foreign Genes", Gene 9: 27 (1980).
Mackie et al., "Tandem Promoters in the Gene for Ribosomal Protein S20 ", J. Biol. Chem. 258: 7840 (1983).
Valenzuela et al., "Nucleotide Sequence of the Gene Coding for the Major Protein of Hepatits B Virus Surface Antigen", Nature 280: 815 (1979 ).
Fritsch et al.; C. R. Acad. Sci. Paris 287 (Series D): 1453 (1978).
Galibert et al.; Nature 281: 646 (1979).
Burrell et al.; Nature 279: 43 (1979).
Stauffer et al.; Plasmid 2: 498 (1979).
Hallewell et al.; Gene 9: 27 (1980).
Valenzuela et al.; Nature 280: 815 (1979).
Alwine, J. C., et al., "Method of Detection of Specific RNAs in Agarose Gels by Transfer to Diazobenzyloxymethyl-Paper and Hybridization with DNA Probes", Proc. Natl. Acad. Sci. 74, 5350 (1977).
Beggs, J. D., "Abnormal Expression of Chromosomal Rabbit Betaglobin Gene in Saccharomyces cerevisiae", Nature, vol. 283, 835 (1980).
Blumberg, B. S., "Australian Antigen and the Biology of Hepatitis B", Science, vol. 197, 17 (1977).
Bolivar, F., "Construction and Characterization of New Clonging Vehicles: III Derivatives of Plasmid pBR322 . . . "]Gene, 4:121 (1977).
Bolivar, F., "Construction and Characterization of New Cloning Vehicles: II A Multipurpose Cloning System", Gene, 4:95, (1977).
Boyer, H. W. and Rolland-Dussiox, "A Complementation Analysis of the Restriction and Modification of DNA in Escherichia coli , Journal of Molecular Biology,"vol. 41, 459-472 (1969).
Charnay, P., et al., "Localization on the Viral Genome and Nucleotide Sequence of the Gene Coding for the Two Major Polypeptides of the Hepatitis B Surface Antigen (HBsAg)", Nucleic Acids Research, vol. 7, 2 (1979).
Christman, J. K., "Amplification of Expression of Hepatitis B Surface Antigen in 3T3 Cells Contransfected with a Dominant-Acting Gene and Cloned Viral DNA", Proc. Natl. Acad. Sci.-USA, vol. 79, 1815 (1982).
Crick, F. H. C., "Split Genes and RNA Splicing", Science, vol. 204, 264 (1979).
Datta, N., "The Purification and Properties of the Penicillinase Whose Synthesis is Mediated by an R-Factor in E. coli", Chem. Abstracts, 64:8558h (1966).
DuBois, M. -F., et al., "Excretion of Hepatitis B Surface Antigen Particles from Mouse Cells Transformed with Cloned Viral DNA", Proc. Natl. Acad. Sci., vol. 77, 4549 (1980).
Edman, et al., "Integration of Hepatitis B Virus Sequences and Their Expression in a Human Hepatoma Cell", Nature (London) vol. 286 (5772), 535-(1980).
Enger-Valk, B. E., et al., "Construction of New Cloning Vehicles with Genes for Tryptophan Operon of E. coli. as Genetic Markers", Abstract, Chem. Abstr. 92:194275m (1980).
Guarente, L., et al., "A Technique for Expressing Eukaryotic Genes in Bacteria", Science, 209, 1428 (1980).
Itakura, K., et al., "Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin", Science, vol. 198, 1056 (1977).
Macnab, G. M., et al., "Hepatitis B Surface Antigen Produced by a Human Hepatoma Cell Line", Journal of Cancer, vol. 34, 509 (1976).
Maxam, A. M., "A New Method for Sequencing DNA", Proc. Natl. Acad. Sci.-USA, vol. 74, 560 (1977).
Mercereau-Puijalon. O., et al., "Synthesis of an Ovalbumin-Like Protein by Escherichia coli K12 Harbouring a Recombinant Plasmid", Nature, vol. 275, 505 (1978).
O'Farrell, P. H., et al., "Regulated Expression by Read-Through Translation from A Plasmid-Encoded Beta-Galactosidase" J. Bact., 134, 645 (May 1978).
Shih, J. W., et al., "Proteins of Hepatitis B Surface Antigen", Journal of Virology, 21, 347 (Jan. 1977).
Shih, J. W., et al., "Proteins of Hepatitis B Surface Antigen: Amino Acid Compositions of the Major Polypeptides", Journal of Virology, 21, 1219 (Mar. 1977).
Silhavy, T. J., et al., "Conversion of Beta-Glactosidase to a Membrane-Bound State by Gene Fusion", Proc. Natl. Acad. Sci., U.S.A. 73, 3423 (Oct. 1976).
Skelly, J., et al., "Hepatitis B Polypeptide Vaccine Preparation in Micelle Form", Nature, vol. 290, 51 (1981).
Southern, E. M., "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis", Journal of Molecular Biology, vol. 98, 503 (1975).
Villa-Komaroff, L., et al., "A Bacterial Clone Synthesizing Proinsulin", Proc. Natl. Acad. Sci., vol. 75, 3727 (1978).
Helling et al. "The Molecular Cloning of Genes", in Genetic Engineering, Chakrabarty (ed.), CRC Press, West Palm Beach, Fla., 1978, pp. 1-30.
Charnay, P., et al, "Brosynthesis of Hepatits B Virus Surface Antigen in Escherichia coli", Nature 286, 893 (1980).
Edman, J. C., et al., "Synthesis of Hepatitis B Surface and Core Antigens in E. coli", Nature 291, 503 (1981).
Pasek, M., et al., "Hepatitis B Virus Genes and their Expression in E. coli" Nature 282, 575 (1979.
Peterson, D. L. et al., "Partial Amino Acid Sequence of Two Major Component Polypeptides of Hepatitis B Surface Antigen", Proc. Natl. Acad. Sci. U.S.A., 74, 1530 (1977).
Peterson, D. L. et al. "Characterization of Polypeptides of HBsAg for the Proposed `UC Vaccine` for Hepatitis B", in Viral Hepatitis: A Contemporary Assessment Etiology, Epidemiology, Pathogenesis and Prevention, Vyas, GN et al. eds, Franklin Negt. Press, Philadelphia, 1978, pp. 569-573.

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