Amplified DNA assay using a double stranded DNA binding protein

Details Amplified DNA assay using a double stranded DNA binding protein Amplified DNA assay using a double stranded DNA binding protein

1994-04-18

1996-07-16

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

536 2433, 435 6, 435 71, 435975, 935 77, C12P 1934, C07H 2104

Patent

active

055366481

ABSTRACT:
A method for capturing amplified target DNA on a solid substrate comprising incorporating a ligand into said DNA by a polymerase chain reaction using a set of primers wherein one of the primers bears the ligand, and contacting the so treated DNA with a solid substrate having a binding reagent for said ligand immobilized thereon.

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Triglia et al. (1990) "Colourimetric detection of PCR products using the DNA binding protein TyR", Nucl. Acids Res. 18(4): 1080.
Goldstein et al. (1976) "The Chemistry of Enzyme Immobilization", in Applied Biochemistry and Bioengineering, vol. 1-Immobilized Enzyme Principles, Wingard et al., eds., Academic Press, pp. 23-126.

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