Amplified assay for analyte

Details Amplified assay for analyte Amplified assay for analyte

1994-08-12

1996-10-22

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 795, 435810, 435969, 436518, 436528, 436541, 436829, G01N 33538

Patent

active

055675913

ABSTRACT:
An indirect assay for analyte employs a particle derivatized with a plurality of molecules of one or more compounds to increase assay sensitivity. In an indirect sandwich assay format, at least one of the compounds is a binder for the analyte, and the tracer is comprised of a binder for at least one of the compounds on the particle. In this manner, a plurality of tracer molecules may be indirectly bound to a single analyte molecule which is bound in a complex formed in the assay.

REFERENCES:
patent: 4193983 (1980-03-01), Ullman et al.
patent: 4762915 (1988-08-01), Kung et al.
patent: 4920046 (1990-04-01), McFarland et al.
patent: 5369036 (1994-11-01), Mercolino

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