Amplification processes

Details Amplification processes Amplification processes

1994-02-23

1996-06-11

Elliott, George C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

536 2433, C12P 1934, C07H 2104

Patent

active

055254942

ABSTRACT:
The present invention relates to a method for the amplification of a target nucleotide sequence using a first primer comprising 1) a target binding nucleotide moiety which is substantially complementary to the desired portion of the target nucleotide sequence; and 2) a polynucleotide tail. The first primer is such that while an extension product thereof may itself serve as a template for primer extension to form an amplification primer extension product, formation of an amplification primer extension product comprising a sequence complementary to the polynucleotide tail is inhibited.

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patent: 4683202 (1987-07-01), Mullis
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Nucl. Acid Res. 12: 4539-4557 (1984).
Nucl. Acid Res. 17: 2503-2516 (1989).
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Keller et al, "Detection of Hepatitis B Virus DNA in Serum by Polymerase Chain Reaction Amplification and Microtiter Sandwich Hybridization", Journal of Clinical Microbiology, Jun. 1990, pp. 1411-1416.
Kemp et al, "Colorimetric Detection of Specific DNA Segments Amplified by Polymerase Chain Reactions", Proc. Natl Acad Sci USA, vol. 86, pp. 2423-2427, Apr. 1989.
Syvanen, et al, "Fast quantification of Nucleic Acid Hybrids by Affinity-Based Hybrid Collection", Nucleic Acids Research, vol. 14, No. 12, 1986, pp. 5037-5048.
Syvanen et al, "Quantification of Polymerase Chain Reaction Products by Affinity-Based Hybrid Collection", Nucleic Acids Research, vol. 16, No. 23, 1988.
Saiki et al, "Genetic analysis of amplified DNA with Immobilized Sequence-Specific Oligonucleotide Probes", Proc. Natl. Acad. Sci. USA, vol. 86, pp. 6230-6234, Aug. 1989, Genetics.
Ng et al, "Recognition and Binding of Template-Primers Containing Defined Abasic Sites by Drosophila DNA Polymerase .alpha. Holoenzyme", The Journal of Biological Chemistry, vol. 264, No. 22, Aug. 5, pp. 13018-13023, 1989.
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