Amplification process

Details Amplification process Amplification process

2005-10-04

2005-10-04

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S194000, C536S023200, C536S023700

Reexamination Certificate

active

06951744

ABSTRACT:
A method for conducting a nucleic acid amplification reaction, said method comprising forming an amplification reaction mixture in the presence of sufficient of a pyrophosphate salt to prevent primer extension taking place, digesting said pyrophosphate salt with a pyrophosphatase enzyme (PPase), and subjecting said reaction mixture to conditions such that an amplification reaction may proceed.This can be used as a “hot start” amplification.Particular novel pyrophosphatase enzymes for use in the method are also described and claimed.

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