Amplification of nucleic acids

Details Amplification of nucleic acids Amplification of nucleic acids

1996-12-02

2000-09-05

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 9121, C12P 1934

Patent

active

06114150&

ABSTRACT:
Methods and compositions are provided to obtain uniform amplification of nucleic acid templates having varied G+C contents by adding betaine and DMSO to the reaction mixture.

REFERENCES:
patent: 5436149 (1995-07-01), Barnes
patent: 5512462 (1996-04-01), Cheng
patent: 5545539 (1996-08-01), Miller
patent: 5545540 (1996-08-01), Mian
patent: 5773213 (1998-06-01), Gullans et al.
Kuebler et al. (1995) Biochemica 4:28-9 (abstract).
Baskaran et al. (1996) Genome Methods 6:633-638.
Pomp et al. (1991) BioTechniques 10(1):58-59.
Barnes, Wayne M. (1994), "PCR Amplification of up to 35-kb DNA with High fidelity and High Yield from .lambda. Bacteriophage Templates," Proc. Natl. Acad. Sci. USA, vol. 91, pp. 2216-2220.
Chong, Samuel S. et al. (1994), "Robust Amplification and Ethidium-Visible Detection of the Fragile X Syndrome CGG Repeat Using Pfu Polymerase," American Journal of Medical Genetics vol.51:522-526.
Rees, William A. et al. (1993), "Betaine Can Eliminate the Base Pair Composition Dependence of DNA Melting," Biochemistry, vol. 32:137-144.

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