Amplification of nucleic acid sequences using oligonucleotides o

Details Amplification of nucleic acid sequences using oligonucleotides o Amplification of nucleic acid sequences using oligonucleotides o

1990-07-13

1992-04-21

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 91, 435810, 436501, 436 94, 935 77, 935 78, C12Q 168, C12P 1934, G01N 3348, G01N 33566

Patent

active

051067277

ABSTRACT:
According to this invention, a process for substantially amplifying template nucleic acid present in a sample is described, wherein said amplification may be performed without prior knowledge of specific sequences, which process comprises apposition of random oligonucleotide primers to said template nucleic acid under conditions such that extension products of said primers are synthesized which are complementary to said template nucleic acid.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
Maniatis et al., Molecular Cloning: A Lab. Manual, Cold Spring Harbor, N.Y. (1982).
Shibata et al., J. Exp. Med. 167:225-230, Jan. 1988).

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