Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1994-12-09
1999-09-28
Zitomer, Stephanie W.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 435 911, 435 5, 935 77, 935 78, C07H 2104, C12P 1934, C12Q 170, C12Q 168
Patent
active
059590955
ABSTRACT:
New methods are provided for the amplification of a midivariant DNA containing an inserted target specific nucleic acid sequence(s) to enable detection of the presence of a target nucleic acid sequence in a test sample. One method employs midivariant DNA as a template for the synthesis of RNA catalyzed by QB replicase. Two midivariant DNA/probe conjugates including a nonreplicable portion of midivariant DNA and a target specific nucleic acid sequence (probe) are described. An amplification method including the steps of hybridization and ligation of the midivariant DNA/probe conjugates followed by replication of the DNA template has enabled detection of less than 200 template molecules. In a modified amplification method one of the midivariant DNA/probe conjugates further includes a RNA polymerase promoter sequence to enable transcription of the midivariant DNA template into RNA. The sequential ligation-transcription-replication method enables the detection of a single template molecule. The detectable molecule(s) are indicative of the presence of the target nucleic acid sequences in the test sample.
REFERENCES:
patent: 4786600 (1988-11-01), Kramer et al.
patent: 4883750 (1989-11-01), Whiteley et al.
patent: 5112734 (1992-05-01), Kramer et al.
Lizardi et al; Bio/Technology 6(10):1197-1202; (Oct. 1988).
Cahill et al, Clin. Chem 37(9), 1482-1485 (1991) "Polymerase Chain Reaction and QB Replicase Amplification".
Feix et al, Febs Lett 63(1), 201-204 (Mar., 1976).
Kramer et al, Nature 339, 401-402 (Jun. 1, 1989) "Replicable RNA Reporters" .
Donahue Jeffrey J.
Martinelli Richard A.
Unger John T.
Blackburn Robert P.
Chiron Diagnostics Corporation
Morgenstern Arthur S.
Zitomer Stephanie W.
LandOfFree
Amplification of midivariant DNA templates does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Amplification of midivariant DNA templates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Amplification of midivariant DNA templates will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-704755