Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-11-02
1995-03-14
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 935 77, 935 78, C12Q 168, C12P 1934
Patent
active
053976983
ABSTRACT:
A method is disclosed for producing multiple copies of a primary polynucleotide sequence located at the 3' terminus of a polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and template-dependent polynucleotide polymerase an extension of a primary polynucleotide sequence hybridized with a template sequence of a single stranded pattern polynucleotide comprising two or more template sequences each containing one or more site specific cleavage sequences, (b) cleaving into fragments said extension at cleavable polynucleotide sequences in the presence of means for specifically cleaving said cleavable polynucleotide sequences when said extension is hybridized with said site specific cleavage sequences, (c) dissociating said fragments, (d) hybridizing said fragments with single stranded pattern polynucleotide, and repeating steps (a)-(d). Steps (a)-(d) may be conducted simultaneously or wholly or partially sequentially. The method may be applied in the detection of a polynucleotide analyte in a sample suspected of containing such analyte to facilitate such detection. Also disclosed are compositions for conducting the method of the invention.
REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4423153 (1983-12-01), Ranney et al.
patent: 4480040 (1984-10-01), Owens et al.
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4490472 (1984-12-01), Gottlieb
patent: 4656134 (1987-04-01), Ringold
patent: 4663283 (1987-05-01), Kleid et al.
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4808525 (1989-02-01), McClelland et al.
patent: 4948731 (1990-08-01), Gehrke et al.
Brigati, et al., Virology, (1983) vol. 126: pp. 32-50 "Detection of Viral Genomes in Cultured Cells and Paraffin-Embedded Tissue Sections Using Biotin-Labeled Hybridization Probes".
Langer, et al., Proc. Natl. Acad. Science USA, (Nov. 1981) vol. 78:11 pp. 6633-6637 "Enzymatic synthesis of biotin-labeled polynucleotides: Novel nucleic acid affinity probes".
Saiki, et al. Science, (Dec. 1985) vol. 230: pp. 1350-1354 "Enzymatic Amplification of .beta.-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia".
Chu, et al., Chemical Abstracts, (Jan. 12, 1987) vol. 106:2, Abstract No. 15257v, "Synthesis of an amplifiable reporter RNA for bioassays".
Maniatis, et al., Molecular Cloning-A Lab Manual, Cold Spring Harbor Lab., (1982) (cited by PTO in its entirety).
Becker Martin
Goodman Thomas C.
Rose Samuel
Ullman Edwin F.
Leitereg Theodore J.
Peries Rohan
Syntex (U.S.A.) Inc.
Zitomer Stephanie W.
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