Amplification method for a polynucleotide

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 912, 435 915, 435 9152, 536 243, 536 2433, 536 253, 536 254, 422 63, 422 681, 422149, 422188, 422189, C12P 1934, C07H 2104, G01N 1506, B01J 804

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059167769

ABSTRACT:
The nucleic acid amplification procedures of the present invention provide methods for amplification of a nucleic acid comprised of a first strand, comprising (a) using the first strand to generate copies of a second strand at a first location, (b) moving the copies of the second strand to a second location, and (c) using the copies of the second strand to generate copies of at least a portion of the first strand. Target nucleic acids used in the context of the present method include RNA or DNA, either single stranded or double stranded, using primer extension or joining-type protocols. Embodiments are set forth for automated forms of the claimed procedures.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 5525494 (1996-06-01), Newton
patent: 5616465 (1997-04-01), Lucas
patent: 5686271 (1997-11-01), Mian et al.
patent: 5726026 (1998-03-01), Wilding et al.
Wu et al. The ligation amplification reaction (LAR)--Amplification of specific DNA sequences using sequential rounds of template-dependent ligation Genomics vol. 4, p. 560-569 1989.

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