Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-11-22
1999-02-09
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
058692371
ABSTRACT:
A method is disclosed for determining the chromosomal identity of a sample of genomic DNA. The genomic DNA is amplified and labeled by polymerase chain reaction using primers substantially complementary to interspersed repetitive DNA sequences. The amplified and labeled genomic DNA fragments are then contacted with chromosomal DNA of known identity under conditions in which the chromosome-specific, but not the interspersed repetitive DNA sequences, of the amplified and labeled genomic DNA fragments are available for hybridization. Specific hybridization to chromosomal DNA of known identity determines the chromosomal identity of the sample of genomic DNA.
REFERENCES:
Cotter, F.E. et al., "Rapid Isolation of Human Chromosome-Specific DNA Probes from a Somatic Cell Hybrid" Genomics 7:257-263 (1990).
Cremer, T. et al., "Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84" Hum. Genet. 74:346-352 (1986).
Cremer, T. et al., "Rapid Interphase and Metaphase Assessment of Specific Chromosomal Changes in Neuroectodermal Tumor Cells by in Situ Hybridization with Chemically Modified DNA Probes" Exp. Cell Res. 176:199-220 (1988).
Gray, J.W., et al., "Fluorescence Hybridization to Human Chromosome 21 Using Probes From a Charon 21A Library" Abstracts for the XII International Meeting of the Society for Analytical Cytology, Cambridge, England, 9-15 Aug. 1987, Cytometry Suppl.1:4, Abstract No. 19.
Landegent, J.E. et al., "Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization" Hum. Genet. 77:366-370 (1987).
Ledbetter, S.A. et al., "PCR-Karyotype' of Human Chromosomes in Somatic Cell Hybrids" Genomics 8:614-622 (1990).
Ledbetter, S.A. et al., "Rapid Isolation of DNA Probes within Specific Chromosome Regions by Interspersed Repetitive Sequence Polymerase Chain Reaction" Genomics 6:475-481 (1990).
Lichter, P. et al., "Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries" Hum. Genet. 80:224-234 (1988).
Litt, M. and R.L. White, "A highly polymorphic locus in human DNA revealed by cosmid-derived probes" PNAS USA 82:6206-6210 (1985).
Nelson, D.L. et al., "Alu polymerase chain reaction: A method for rapid isolation of human-specific sequences from complex DNA sources" PNAS USA 86:6686-6690 (1989).
Pinkel, D. et al., "Detection of structural chromosome abberations in metaphase spreads and interphase nuclei by in situ hybridization high complexity probes which stain entire human chromosomes" Am. J. Hum. Genet. 43 Suppl.3: A118, Abstract No. 0471 (1988).
Sealy, P.G. et al., "Removal of repeated sequences from hybridisation probes" Nuc. Acids Res. 13(6):1905-1922 (1985).
Soeiro, R. and J.E. Darnell, "Competition hybridization by `pre-saturation` of HeLa cell DNA" Chemical Abstracts 71(25):195, Abstract No. 121640h (1969).
Soeiro, R. and J.E. Darnell, "Competition Hybridization by `Pre-saturation` HeLa Cell DNA" J. Mol. Biol. 44:551-562 (1969).
Lichter Peter
Ward David C.
Elliott George C.
Hanley Elizabeth A.
Kara Catherine J.
Larson Thomas G.
Yale University
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