Amplification and detection of mycobacterium avium complex speci

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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536 231, 536 243, C12Q 168, C07H 2104

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active

056817053

ABSTRACT:
Amplification primers and methods are disclosed for complex-specific amplification of target sequences in the dnaJ genes of the Mycobacterium Avium Complex species. Also provided are assay probes for detection of the amplification products and/or identification of the MAC species which is present.

REFERENCES:
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Uematsu et al., J. of the Japanese Assoc. for Infect. Dis. 67(5): 421-428 (1993). Abstract only Bej et al., Critical Reviews in Biochemistry and Molecular Biology 26 (3/4): 301-334(1991).
G. T. Walker, et al. "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polylmerase system" Proc. Natl. Acad. Sci. USA 89:392-396 (1992).
G. T. Walker, et al. "Strand displacement amplification--an isothermal, in vitro DNA amplification technique" Nucl. Acids Res. 20:1691-1696 (1992).
S. I. Takewaki, et al. "Nucleotide sequence comparison of the Mycobacterial dnaJ gene and PCR-restriction fragment length polymorphism analysis for identification of mycobacterial species" Int. J. Syst. Bact. 44:159-166 (1994).
S. I. Takewaki, et al. "Genus-specific Polymerase Chain Reaction for the Mycobacterial dnaJ gene and species-specific oligonucleotide probes" J. Clin. Microbiol. 31:446-450 (1993).
R. B. Lathigra, et al. "A gene from Mycobacterium tuberculosis which is homologous to the DnaJ heat shock protein of E. coli" Nucl. Acids Res. 16:1636 (1988).

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