Amplification and detection of mycobacterium avium complex speci

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 911, 435 6, 536 243, C12P 1934

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056679947

ABSTRACT:
Amplification primers and methods for complex-specific amplification of target sequences in the dnaJ genes of the Mycobacterium avium Complex (MAC) species are disclosed. The primer target binding sequences are useful for amplification of the dnaJ target in a variety of amplification reactions, with detection of the complex-specific target and, optionally, identification of the MAC species from which the target is derived. Primers and methods for multiplex amplification of dnaJ and a second target are also described.

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S. Takewaki, et al. "Nucleotide Sequence Comparison of the Mycobacterial dnaJ Gene and PCR-Restriction Fragment Length Polymorphism Analysis for Identification of Mycobacterial Species" Int. J. Sys. Bact. 44:159-166 (1994).
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R. Lathigra, et al. "A gene from Mycobacterium tuberculosis which is homologous to the Dna J heat shock protein of E. coli" Nucl. Acids Res. 16:1636 (1988).
S. Takewaki, et al. "Genus-Specific Polymerase Chain Reaction for the Mycobacterial dnaJ Gene and Species-Specific Oligonucleotide Probles" J. Clin. Microbiol. 31:446-450 (1993).

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