Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-05-10
1998-03-03
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435912, 536243, 536 2431, 536 2432, 536 2433, C12Q 168, C12Q 170, C12P 1934, C07H 2104
Patent
active
057232962
ABSTRACT:
Methods for identification or detection of a species of an organism or a group of related species of an organism by species non-specific amplification of a target sequence followed by species- or group-specific detection of the amplification products. Also provided are a target sequence which is amplifiable in multiple species of mycobacteria using a single pair of amplification primers and species- and group-specific detector probes for hybridization to the assay regioin of the amplified target. Blocking oligonucleotides are employed to allow discrimination among species in which the amplified target sequences are sufficiently similar that they cross-hybridize to an assay probe.
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Nadeau James G.
Nycz Colleen Marie
Scott Patricia Brinkley
Shank Daryl Dee
Spears Patricia Anne
Becton Dickinson and Company
Fredman Jeffrey
Fugit Donna R.
Jones W. Gary
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