Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1988-09-12
1989-05-30
Niebling, John F.
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
204299R, 2041832, G01N 2728, G01N 2726, G01N 2740
Patent
active
048348627
ABSTRACT:
An ampholyte separation apparatus and method which utilize a thermally engendered pH gradient to separate amphoteric molecules according to their isoelectric points and/or mobilities. Two independently temperature controllable thermal masses located on opposing ends of a plurality of barriers situated transverse to a flow channel create a pH gradient along the length of the transverse barriers. A pair of electrodes create an electric field along the length of the transverse barriers so as to cause ampholytes traveling down the flow channel to migrate into the transverse barriers and stabilize at points corresponding to their isoelectric points. Alteration of the pH gradient causes the separated amphoteric molecules to reenter the flow stream at different times and to be separately eluted and collected.
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Breiner Steven J.
Lochmuller Charles H.
Duke University
Jenkins Richard E.
Niebling John F.
Starsiak Jr. John S.
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