Ammonium substituted chemiluminescent labels and their conjugate

Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...

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436501, 530409, 544355, 546 61, 546 71, 546 93, 546102, 546107, 546108, 546112, 546147, 546170, 5483094, C07D21904

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active

052817121

ABSTRACT:
A novel chemiluminescent labeling compositions comprising an ester or thiolester covalently and jointly bonded to (1) a carbon of a heterocyclic ring or ring system that is susceptible to attack by peroxide or molecular oxygen and (2) an aryl ring or ring system wherein the heterocyclic ring or ring system is distinquished by a heteroatom thereof in an oxidation state which causes the attacked carbon atom to form an intermediate that decays and produces chemiluminescence; the aryl ring or ring system contains at least three substituents on a six-member aromatic hydrocarbon that together sterically and electronically hinder hydrolysis of the linkage, which substituents involve ortho substituent groups on the aryl in conjunction with quaternary ammonium meta or para substituents thereon. Included are the chemiluminescent labeling composition conjugated with a specific binding material; a chemiluminescent assay comprising the conjugate; and a chemiluminescent assay kit comprising the conjugate with the capability of conducting the assay.

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patent: 4745181 (1988-05-01), Law et al.
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patent: 4946958 (1990-08-01), Campbell et al.
patent: 4950613 (1990-08-01), Arnold et al.
patent: 5110932 (1992-05-01), Law et al.

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