Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-08-15
1996-12-03
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104
Patent
active
055807275
ABSTRACT:
Means for diagnosing t(8;21) translocation type acute myeloid leukemia with high sensitivity by a simple operation are disclosed. The present invention provided AML1-MTG8 fused DNA having the nucleotide sequence shown in SEQ ID No. 1 and DNA fragments thereof containing the fused site. The present invention provided AML1-MTG8 fused polypeptide having the amino acid sequence shown in SEQ ID No. 2, and fragments thereof containing the fused site. The present invention provided AML1-MTG8 fused mRNA having the same nucleotide sequence as shown in SEQ ID No. 1 except that thymine is replaced with uracil, and fragments thereof. The present invention provided a probe which is said DNA fragment that is labelled. The present invention provided a method for detecting said fused DNA by using said probe. The present invention provided a method for detecting AML1-MTG8 fused mRNA or a fragment thereof in a sample, comprising the steps of amplifying said fused DNA or a fragment thereof by a nucleic acid-amplifying method and detecting the amplified DNA fragment.
REFERENCES:
patent: 4683202 (1987-07-01), Mullis
Erickson et al, (Oct. 1992), "Identification of breakpoints in t(8;21) acute myelogenous leukemia and isolation of a fusion transcript, AML1/ETO, with similiarity to Drosophila segmentation gene, runt", Blood 80(7):1825-1831.
Miyoshi et al, (1991), "T(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1"proc. Natl. Acad. Sci. 88:10431-10434.
Mifflin et al, (1989) "Use and applications of nucleic acid probes in the clinical laboratory"Clin. Chem. 35(9):1819-1825.
Kozu et al, (Aug. 1993), "Junctions of the AML1/MTG8(ETO) fusion are constant in t(8;21) acute myeloid leukemia detected by reverse transcription polymerase chain reaction", Blood 82(4):1270-1276.
Berger et al, (1982), "Cytologic characterization and significance of normal karyotypes in t(8;21) acute myeloblaslic leukemia", Blood 59(1):171-178.
Kozu et al, (1993), "Identification of AML1-MTG8 fusion transcripts generated by the chromosomal translocation t(8;21) in human acute myeloid leukemia", J. Cell. Biochem. Suppl. 17A:215.
Maseki et al, (Mar. 1993), "The 8;21 chromosomes translocation in acute myeloid leukemia is always detectable by molecular analysis using AML1", Blood 81 (6):1573-1579.
Miyoshi et al, (Jul. 1993), "The t(8;21) translocation in acute myeloid leukemia results in production of an AML1-MTG8 fusion transcript", EMBO J. 12(7):2715-2721.
Shimizu et al, (Dec. 1992), "Consistent disruption of the AML1 gene ocurs within a single intron in the t(8;21) chromosomal translocation", Cancer Res. 52:6945-6948.
Kikuchi Kimiko
Kozu Tomoko
Miyoshi Hiroyuki
Ohki Misao
Fredman Jeffrey
SRL, Inc.
Zitomer Stephanie W.
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