Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Scleroproteins – e.g. – fibroin – elastin – silk – etc.
Patent
1998-03-02
2000-07-18
Johnson, Nancy A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Scleroproteins, e.g., fibroin, elastin, silk, etc.
530350, 530300, 530344, 530412, 530413, 530417, 530402, C07K 1478, C07K 114, C07K 14435
Patent
active
06090920&
ABSTRACT:
Aminoterminal propeptide of type I procollagen exists in serum in two forms: classical type I procollagen which is a heterotrimer containing two pro .alpha.1-chains and one pro .alpha.2-chain of type I procollagen, and an .alpha.1-homotrimer type I procollagen containing three identical pro .alpha.1-chains. These intact, trimeric aminoterminal propeptides may be isolated without the use of proteolytic enzymes and the resultant propeptides may be used to prepare antibodies specific for the intact trimeric propeptide, having no affinity for the monomeric form of the propeptide. Such antibodies are useful in methods of assaying intact trimeric aminoterminal propeptide of type I procollagen in serum, without false information resulting from inadvertent assay of the monomeric form of the propeptide.
REFERENCES:
Sambrook, J et al. Molecular cloning: a laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, 1989.
Eberling, Peter R., et al., "Utility of Type I Procollagen Propeptide Assays for Assessing Abnormalities in Metabolic Bone Diseases", Journal of Bone and Mineral Research, vol. 7, No. 11, pp. 1243-1250 (1992).
Jukkola, Arja, et al., "Procollagen Synthesis and Extracellular Matrix Deposition in MG-63 Osteosarcoma Cells", Journal of Bone and Mineral Research, vol. 8, No. 6, pp. 651-657 (1993).
Kohler, G., et al., "Continuous cultures of fused cells secreting antibody of predefined specificity", Nature, vol. 256, pp. 495-497 (1975).
Linkhart, Susan G., et al., "Synthetic Peptide-Based Immunoassay for Amino-Terminal Propeptide of Type I Procollagen: Application for Evaluation of Bone Formation", Clin. Chem. vol. 39, No. 11, pp. 2254-2258 (1993).
Mellko, Jukka et al., "Radioimmunoassay of the Carboxyterminal Propeptide of Human Type I Procollagen", Clin. Chem., vol. 36, No. 7, pp. 1328-1332 (1990).
Price, Karen M., et al., "Development of a radioimmunoassay for fetal antigen 2", Clin. Chem. Acta., vol. 224, pp. 95-102 (1994).
Skerra, Arne, et al., "Assembly of a Functional Immunoglobulin F.sub.v Fragment in Escherichia coli", Science, vol. 240, pp. 1038-1041 (1988).
Teisner, B., et al., "Fetal antigen 2: An amniotic protein identified as the aminopropeptide of the .alpha.1 chain of human procollagen type I", APMIS, vol. 100, pp. 1106-1114 (1992).
Frei et al., Hepatology, vol. 4, pp. 830-834 (1984).
Rohde et al., Fresenius Z., Anal. Chem. vol. 290, pp. 151-152 (1978).
Mellko et al., Clin. Chem., vol. 36, No. 7, pp. 1328-1332 (1990).
Taubman et al., Science, vol. 186, pp. 1115-1117 (1974).
Pesciotta et al., Biochemistry, vol. 19, pp. 2447-2454 (1980).
Foellmer et al., Eur. J. Biochem., vol. 134, pp. 183-189 (1983).
Kauppila Saila
Melkko Jukka
Risteli Juha
Risteli Leila
Holleran Anne L.
Johnson Nancy A.
Orion-yhtyma Oy
LandOfFree
Aminoterminal propeptides of typed 1 procollagen does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Aminoterminal propeptides of typed 1 procollagen, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Aminoterminal propeptides of typed 1 procollagen will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2038041