Aminosilane/carbodiimide coupling of DNA to glass substrate

Details Aminosilane/carbodiimide coupling of DNA to glass substrate Aminosilane/carbodiimide coupling of DNA to glass substrate

1997-05-13

1998-06-02

Nutter, Nathan M.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

523112, 604266, 424409, 424422, C08G 6348, C08G 6391

Patent

active

057601305

ABSTRACT:
A method for coupling DNA to a glass substrate by aminating the glass substrate with an aminosilane, reacting DNA with a carbodiimide/imidazole solution to create a 5'-phosphorimidazolide, and reacting the aminated glass substrate and phosphorimidazolide to couple the DNA to the substrate.

REFERENCES:
patent: 5350800 (1994-09-01), Verhoeven et al.
Guo et al., "Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports", 22 Nucleic Acids Research 5456 (1994).
Maskos et al. "Oligonucleotide hybridisations on glass supports: a novel linker for oligonucleotide synthesis and hybridisation properties of oligonucleotides synthesised in situ", 20 Nucleic Acids Research 1679 (1992).
Eggers et al., "A Microchip for Quantitative Detection of Molecules Utilizing Luminescent and Radioisotope Reporter Groups", 17 BioTechniques 516 (1994).
Chu et al., "Derivatization of unprotected polynucleotides", 11 Nucleic Acids Research 6513 (1983).

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