Aminopeptidase derived from bacillus licheniformis , gene...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S068100, C435S069100, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

07098018

ABSTRACT:
The present invention relates to novel aminopeptidase derived fromBacillus licheniformis,a gene encoding the aminopeptidase, an expression vector containing the gene, a cell transformant transfected with the expression vector and a process for preparing a natural type protein using thereof. More particularly, the present invention relates to a gene encoding aminopeptidase which is cloned and manufactured using the recombinant DNA technique, an expression vector containing the gene, a cell transformant transfected with the expression vector and a recombinant aminopeptidase which is necessary to produce recombinant human growth hormone in a natural type protein and can be expressed in a high yield more stably and advantageously, compared with conventional methods for the purification.

REFERENCES:
patent: 4755465 (1988-07-01), Gray et al.
patent: 5112812 (1992-05-01), Samuelsson et al.
patent: 5126249 (1992-06-01), Becker et al.
patent: 6428997 (2002-08-01), Lee et al.
patent: 2002/0146721 (2002-10-01), Berka et al.
patent: WO 89/12678 (1989-12-01), None
patent: WO 98/38290 (1998-09-01), None

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