Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-08-01
2006-08-01
Le, Long V. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C436S518000, C436S524000, C436S525000, C427S002110, C427S002130, C427S002140, C427S127000, C427S128000, C427S129000, C427S007000, C435S005000, C435S007920, C435S177000, C435S178000, C435S180000, C530S812000, C530S813000, C530S815000, C530S816000
Reexamination Certificate
active
07083936
ABSTRACT:
A composition containing polydisperse aminodextran polymer molecules is soluble in an aqueous solution at a concentration of 10 mg/ml. The molecules therein have a narrow size distribution characterized by an average molecule mean hydrodynamic diameter of greater than 115 nm, a polydispersity index of between 0.10 and 0.47, an average molecular weight (MW) greater than 3 million daltons, and an amine content of greater than 50 amines per molecule. Similar soluble compositions contain the polymer molecules with an average MW of greater than 7 million daltons. These compositions are useful in forming reagents by conjugation with proteins for labeling cells. Methods of making these compositions and reagents from conventional mixtures of aminodextran polymers involve fractionation on column chromatography.
REFERENCES:
patent: 4542104 (1985-09-01), Stryer et al.
patent: 5171846 (1992-12-01), Gupta
patent: 5272257 (1993-12-01), Gupta
patent: 5424297 (1995-06-01), Rubio et al.
patent: 5466609 (1995-11-01), Siiman et al.
patent: 5527713 (1996-06-01), Bolton et al.
patent: 5543332 (1996-08-01), Lihme et al.
patent: 5627078 (1997-05-01), Karl et al.
patent: 5658741 (1997-08-01), Bolton et al.
patent: 5891741 (1999-04-01), Siiman et al.
patent: 5945293 (1999-08-01), Siiman et al.
patent: 5994089 (1999-11-01), Siiman et al.
patent: 6074884 (2000-06-01), Siiman et al.
patent: 6207385 (2001-03-01), Stanley
patent: 6287568 (2001-09-01), Wang et al.
patent: 6387622 (2002-05-01), Siiman et al.
patent: WO 00/07019 (2000-02-01), None
Cairo, C.W., et al., “Control of Multivalent Interactions by Binding Epitope Density”,J. Am. Chem. Soc., 124(8), pp. 1615-1619 (2002).
T.M. Hansen, “Fluorescent dextran conjugates for use in quantitative assays”,IVD Technol., pp. 35-40 (Apr. 2003).
Siiman, et al, “Fluorescent neoglycoproteins: Antibody-Aminodextran-Phycobiliprotein Conjugates”,Bioconjugate Chem., 10(6), pp. 1090-1106 (1999).
Healy Cynthia G.
Ledis Stephen L.
Siiman Olavi
Alter Mitchell E.
Beckman Coulter Inc.
Do Pensee T.
Howson and Howson
Le Long V.
LandOfFree
Aminodextran compositions and conjugates and method of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Aminodextran compositions and conjugates and method of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Aminodextran compositions and conjugates and method of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3678783