Amino acid transporters and uses

Details Amino acid transporters and uses Amino acid transporters and uses

1998-03-17

2000-08-08

Hobbs, Lisa

Chemistry: molecular biology and microbiology

Miscellaneous

435 691, 4353201, 435455, 435471, 4352523, 435325, 530350, C12N 100, C12N 500, C12P 2106, C07K 100

Patent

active

06100085&

ABSTRACT:
The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of the human amino acid transporter proteins EAAT1, EAAT2, EAAT3 and ASCT1. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to each of these transporter genes. Also provided are recombinant expression constructs capable of expressing each of the amino acid transporter genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter proteins encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.

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