Amino acid transporters and uses

Details Amino acid transporters and uses Amino acid transporters and uses

1999-06-14

2000-05-09

Hobbs, Lisa J.

Chemistry: molecular biology and microbiology

Miscellaneous

4353201, 435325, 435 691, 435366, 530350, C12N 100, C12N 500, C12P 2106, C07K 100

Patent

active

060603070

ABSTRACT:
The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of a human amino acid transporter protein termed EAAT4 and genes encoding such a transporter. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to this transporter gene. Also provided are recombinant expression constructs capable of expressing this amino acid transporter gene in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter protein encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.

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