Amino acid transporters and uses

Details Amino acid transporters and uses Amino acid transporters and uses

1995-10-23

1999-07-06

Wax, Robert A.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

4352523, 4353201, 536 235, 536 243, C12N 500, C12N 120, C12N 1500, C07H 2104

Patent

active

059196998

ABSTRACT:
The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of the human amino acid transporter proteins EAAT1, EAAT2, EAAT3 and ASCT1. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to each of these transporter genes. Also provided are recombinant expression constructs capable of expressing each of the amino acid transporter genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter proteins encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.

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