Amino acid transporters and uses

Details Amino acid transporters and uses Amino acid transporters and uses

1998-11-09

2000-07-18

Hobbs, Lisa J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 78, 435 29, 435 691, 435 701, 436501, 436504, C12Q 168, G01N 3353, G01N 33566, C12P 2104

Patent

active

060905603

ABSTRACT:
The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of a human amino acid transporter protein termed EAAT4 and genes encoding such a transporter. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to this transporter gene. Also provided are recombinant expression constructs capable of expressing this amino acid transporter gene in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter protein encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 5385831 (1995-01-01), Mulvihill et al.
patent: 5424185 (1995-06-01), Lam et al.
Arriza et al. (1994) J. Neurosci., vol. 14, No. 9, p. 5559-5569.
Kanai et al. (1992) Nature, 360: 467-471.
Kanai et al. (1993) Trends in Neurosci., vol. 16, No. 9, p. 365-370.
Kanai et al., (1993) FASEB J., 7: 1450-1459.
Kanner, (1993), FEBS Lett., 325 (1,2): p. 95-99.
Pines et al., (1992) Nature, 360: p. 464-467.
Schloss et al. (1992) FEBS Lett., 307 (1): p. 76-80.
Shashidharan et al., (1993), Biochim. Biophys. Acta., 1216: p. 161-164.
Stelzner et al., (1993) FASEB J., 7(4/part 2): A575.
Storck et al., (1992), Proc. Natl. Acad. Sci., 89: p. 10955-10959.
Uhl, (1992), Trends in Neurosci., 15(7): 265-268.
Anderson et al., (1989) J. Biol. Chem., 264: p. 8222-822.
Arriza et al., (1992) J. Neurosci., 12: 4045-4055.
Barish, (1983) J. Physiol., 342: 309-325.
Bertling et al., (1987) Bioscience Reports, 7: 107-112.
Blakely et al., (1991) Anal. Biochem., 194: 302-308.
Bouvier et al., (1992) Nature, 360: 471-474.
Bussolati et al., (1992) J. Biol. Chem., 267: 8330-8335.
Choi et al., (1987) Neurosci., 7: 357-358.
Chomczynski & Sacchi, (1987) Anal. Biochem., 162: 156-159.
Christensen (1990), Physiol. Rev., 70: 43: 77.
Christensen et al., (1967), J. Biol. Chem., 242: 5237-5246.
Eisenberg et al., (1984), J. Molec. Biol., 179: 125-142.
Engelke et al., (1992) J. Bacteriol., 171: 5551-5560.
Fairman, (1995) Human Excitatory Amino Acid Transporter 4. Genbank Accession No. U18244.
Felgner et al., (1987) Proc. Natl. Acad. Sci., 84: 7412-7417.
Georgiou, (1988) AICHE Journal, vol. 34, No. 8, pp. 1233-1248.
Gluzman, (1981) Cell, 23: 175-182.
Guastella et al., (1992) Proc. Natl. Sci., 89: 7189-7193.
Guastella et al., (1990) Science, 249: 1303-1306.
Kanai et al., (1994) J. Biol. Chem., vol. 269, No.32, pp. 20599-20606.
Kanner & Schuldiner, (1987), CRC Crit. Rev. Biochem., 22: 1-38.
Kavanaugh et al., (1992) J. Biol. Chem., 267: 22007-22009.
Kim et al., (1991) Nature, 352: 725-728.
Kong et al., (1993) J. Biol. Chem., 268: 1509-1512.
Kozak, (1987) Nucleic Acid Res., 15: 8125-8132.
Maenz et al., (1992), J. Biol. Chem., 267: 1510-1516.
Makowske & Christensen, (1982) J. Biol. Chem., 257: 14635-14638.
Nicholls & Atwell, (1990), TIPS, 11: 462-468.
Olney et al., (1990) Science, 248: 596-599.
Quick and Lester, (1994) Methods in Neuroscience, 19: 261-279.
Saiki et al., (1988) Science, 239: 487-491.
Sanger et al., (1977) Proc. Natl. Acad. Sci., 74: 5463.
Smith & Johnson, (1988) Gene, 67: 31-40.
Smithies et al., (1985) Nature, 317: 230-234.
Thomas & Capecchi, (1987) Cell, 51: 503-512.
Wallace et al., (1990) J. Bacteriol., 172: 3214-3220.
Wang et al., (1991) Nature, 352: 729-731.
Dreyer et al., (1996) Arch. Ophthalmol., 114: 299-305.
Honda (1996) Nippon Ganka Gakkst Zasshi, 100: 937-955.
Kalloniatis, (1995) J. Amer. Optom. Assoc., 66: 750-757.
Zerangue et al., (1995) J. Biol. Chem., 270: 6433-6435.
Kataoka et al., (1997) J. Neurosci., 17: 7017-7024.
Sheng et al., (1996) Neuron., 17: 575-578.

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