Amino acid substituted-cresyl violet, synthetic fluorogenic...

Details Amino acid substituted-cresyl violet, synthetic fluorogenic... Amino acid substituted-cresyl violet, synthetic fluorogenic...

1998-08-05

2001-05-22

Marschel, Ardin H. (Department: 1631)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S023000, C436S172000, C544S199000

Reexamination Certificate

active

06235493

ABSTRACT:

FIELD OF THE INVENTION
The present invention concerns amino acid substituted-cresyl violet compounds such as (Ala-Pro)
2
-cresyl violet and (CBZ-Arg)
2
-cresyl violet, as synthetic fluorogenic, substrates for the analysis of kinetic parameters of enzymes in individual mammalian (i.e., human) cells or tissue.
DESCRIPTION OF RELATED ART
The art is discussed in two main areas. The first area is directed, the background concerning evaluation of the biochemical activity of extracellular cathepsin B in liver tissue. The second area is directed to the synthesis and use of an amino acid-cresyl violet agent to determine the kinetic parameters of dipeptidyl peptidase IV (CD26) in liver tissue.
Evaluation of Extra Cellular Cathepsin B
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