Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1988-06-15
1992-05-26
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 24, 435 25, 435810, C12Q 148, C12Q 137, C12Q 126
Patent
active
051167301
ABSTRACT:
Novel substrates which are amino acid 4-amino phenazones for detecting enzymes, particularly L-gamma-glutamyltransferase, are described. The amino acid gamma-glutamyl-4-aminophenazone reacts with the enzyme to produce 4-aminophenazone which is an amino dye intermediate. The amino dye intermediate is coupled with a second dye intermediate to form a chromogen. The 4-aminophenazone is particularly reacted with a phenolic naphtholic or aniline compound, particularly the phenolic compound 2-hydroxy-3,5-dichlorobenzenesulfonate, to form a red chromogen in the presence of an oxidizing agent, such as bilirubin oxidase.
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Artiss Joseph D.
Bensie Jill M.
Raden Daniel S.
Zak Bennie
Board of Governors of Wayne State University
Kepplinger Esther L.
McLeod Ian C.
Wortman Donna C.
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