Amino acid sequence variant alfalfa antifungal protein and...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Plant proteins – e.g. – derived from legumes – algae or...

Reexamination Certificate

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C530S379000

Reexamination Certificate

active

08067542

ABSTRACT:
The present invention relates to an antifungal protein, AlfAFP1, which is a modified form of an antifungal protein isolated fromMedicagoplants, the modified form exhibiting enhanced anti-fungal activity for controlling fungal pathogenesis in plants. A method for inhibiting fungal colonization of plants is described which includes preparation of nucleotide sequences encoding the modified antifungal protein, preparation of vectors containing the nucleotide coding sequence, and methods for transforming plants with the nucleotide sequences. The polypeptide can be formulated into compositions useful in controlling plant pathogenic fungi.

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DeSamblanx et al. 1997, The Journal of Biological Chemistry 272:1171-1179.
Papadopoulou E, et al.,Critical Area Computation via Voronoi Diagrams, IEEE Transactions on Computer-Aided Design of Integrated Circuits and Systems 18(4):463-474 (1999).
Papadopoulou E, et al.,The Loo Voronoi Diagram of Segments and VLSI Applications, Int. J. Comput. Geom. Appl. 11(5):503-528 (2001).
DeSamblanx et al.,Mutational Analysis of a Plant Defensin from Radish(Raphanus sativus L.)Reveals Two Adjacent Sites Important for Antifungal Activity, J. Biol. Chem. 272: 1171-1179 (1997).

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