Amino acid sequence variant alfalfa antifungal protein and...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance

Reexamination Certificate

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C800S278000, C800S301000, C536S023600

Reexamination Certificate

active

07728190

ABSTRACT:
The present invention relates to an antifungal protein, AlfAFP1, which is a modified form of an antifungal protein isolated fromMedicagoplants, the modified form exhibiting enhanced anti-fungal activity for controlling fungal pathogenesis in plants. A method for inhibiting fungal colonization of plants is described which includes preparation of nucleotide sequences encoding the modified antifungal protein, preparation of vectors containing the nucleotide coding sequence, and methods for transforming plants with the nucleotide sequences. The polypeptide can be formulated into compositions useful in controlling plant pathogenic fungi.

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DeSamblanx et al., Mutational Analysis of a Plant Defensin from Radish (Raphanus sativusL.) Reveals Two Adjacent Sites Important for Antifungal Activity,Journal of Biological Chemistry272:1171-1179 (1997).

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