Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2011-03-22
2011-03-22
Crane, Lawrence E (Department: 1623)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S025340, C536S026800
Reexamination Certificate
active
07910726
ABSTRACT:
The disclosure provides an excellent amidite for synthesizing a modified nucleic acid, which enables a protective group therein to be removed under a moderate condition, thereby stably producing a hydroxyl group-containing modified nucleic acid. The disclosure also provides a method for synthesizing a modified nucleic acid using the amidite. Specifically, an amidite for synthesizing the modified nucleic acid is expressed by General Formula (I):where X represents a base, Y represents a substituent, Z represents a protective group for protecting a hydroxyl group in the substituent, and Q represents one of a hydrogen atom, a hydroxyl group and a hydroxyl group protected by a protective group, wherein the protective group can be removed in an aprotic solvent, and when the protective group is removed, the hydroxyl group emerges in the substituent.
REFERENCES:
patent: WO 03/078623 (2003-09-01), None
Kuijpers, W.H.A. et al; “The 2-(Acetoxymethyl) Benzoyl (AMB) Group As a New Base-Protecting Group, Designed for the Protection of (Phophate) Modified Oligonucleotides.”; Tetrahedron Letters, vol. 31, No. 46, pp. 6729-6732, 1990.
Eritja, Ramon et al; “A Synthetic Procedure for the Preparation of Oligonucleotides Without Using Ammonia and Its Application for the Synthesis of Oligonucleotides Containing O-4-Alkyl Thymidines.”; Tetrahedron, vol. 48, No. 20, pp. 4171-4182, 1992.
Avino, Anna Maria et al.; “Use of NPE-Protecting Groups for the Preparation of Oligonucleotides Without Using Nucleophiles During the Final Deprotection.”; Nucleosides & Nuceotides, vol. 13, No. 10, pp. 2059-2069, 1994.
Kuijpers, W.H.A. et al.; “The application of the AMB protective group in the solid-phase synthesis of methylphosphonate DNA analogues”; Nucleic Acids Research , vol. 21, No. 15, pp. 3493-3500, 1993.
Koole, Leo H. et al; “Synthesis of Phosphate-Methylated DNA Fragments Using 9-Fluorenylmethoxycarbonyl as Transient Base Protecting Group”; J. Org. Chem., vol. 54, pp. 1657-1664, 1989.
Heikkila, Jarmo et al.; “The 9- Fluorenylmethoxycarbonyl (Fmoc) Group for the Protection of Amino Functions of Cytidine, Adenosine, Guanosine and Their 2′-Deoxysugar Derivatives”; Acta Chemica Scandinavica., B37, No. 3, p. 263, 1983.
Crane Lawrence E
Fujitsu Limited
Westerman Hattori Daniels & Adrian LLP
LandOfFree
Amidite for synthesizing modified nucleic acid and method... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Amidite for synthesizing modified nucleic acid and method..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Amidite for synthesizing modified nucleic acid and method... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2684352