Radiant energy – Ionic separation or analysis – With sample supply means
Reexamination Certificate
2005-02-01
2005-02-01
Lee, John R. (Department: 2881)
Radiant energy
Ionic separation or analysis
With sample supply means
C250S281000, C250S42300F, C250S42300F
Reexamination Certificate
active
06849847
ABSTRACT:
A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises:The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising:(a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device;(b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr;(c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure;(d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and(e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.
REFERENCES:
patent: 3944826 (1976-03-01), Gray
patent: 4209697 (1980-06-01), Renner et al.
patent: 4239967 (1980-12-01), Carr et al.
patent: 4259572 (1981-03-01), Brunnee et al.
patent: 4527059 (1985-07-01), Benninghoven et al.
patent: 4686366 (1987-08-01), Stuke
patent: 4740692 (1988-04-01), Yamamoto et al.
patent: 5045694 (1991-09-01), Beavis et al.
patent: 5070240 (1991-12-01), Lee et al.
patent: 5118937 (1992-06-01), Hillenkamp et al.
patent: 5164592 (1992-11-01), Kitamori et al.
patent: 5260571 (1993-11-01), Cottrell et al.
patent: 5300774 (1994-04-01), Buttrill, Jr.
patent: 5580733 (1996-12-01), Levis et al.
patent: 5625184 (1997-04-01), Vestal et al.
patent: 5633496 (1997-05-01), Sakairi et al.
patent: 5663561 (1997-09-01), Franzen et al.
patent: 5917185 (1999-06-01), Yeung et al.
patent: 5945678 (1999-08-01), Yanagisawa
patent: 5965884 (1999-10-01), Laiko et al.
patent: 6259091 (2001-07-01), Eiden et al.
patent: 2299445 (1996-10-01), None
patent: 2310950 (1997-09-01), None
patent: 9428418 (1994-12-01), None
Jun Qin et al., “Matrix-Assisted Laser Desorption Ion Trap Mass Spectrometry: Efficient Isolation and Effective Fragmentation of Peptide Ions,”Anal. Chem.68:2108-2112 (1996).
Jun Qin et al., “Matrix-Assisted Laser Desorption Ion Trap Mass Spectrometry: Efficient Trapping and Ejection of Ions,”Anal. Chem.68:2102-2107 (1996).
V. Laiko et al., “Atmospheric Pressure Matrix Assisted Laser Desorption Ionization,” 4th International Symposium on Mass Spectrometry in the Health and Life Sciences. Poster # 1121, Aug. 25-29, 1998, San Francisco, CA.
A.N.Krutchinsky, et al., “Orthogonal Injection of Matrix-Assisted Laser Desorption/Ionization Ions into a Time-of-flight Spectrometer Through a Collisional Damping Interface,”Rapid Communication in Mass Spectrometry, vol. 12, pp. 508-518 (1998).
D.P. Little, et al., “MALDI on a Chip: Analysis of Arrays of Low-Femtomole to Subfemtomole Quantities of Synthetic Oligonucleotides and DNA Diagnostic Products Dispensed by a Piezoelectric Pipet.”Anal. Chem.(1997), vol. 69, pp. 4540-4546.
J. Qin, et al., “A Practical Ion Trap Mass Spectrometer for the Analysis of Peptides by Matrix-Assisted Laser Desorption/Ionization.”Anal. Chem.(1996), vol. 68, (#10), pp. 1784-1791.
M. Mann et al., “Developments in Matrix-assisted Laser Desorption/Ionization Peptide Mass Spectrometry”,Analytical Biotechnology, vol. 7, pp. 11-19, (1996).
K. Dreisewerd et al., “Influence of the Laser Intensity and Spot Size on the Desorption of Molecules and Ions in Matrix-assisted Laser Desorption/Ionization with a Uniform Beam Profile.”International Journal of Mass Spectrometry and Ion Processes, vol. 141, pp. 127-148 (1995).
B. Spengler et al., “The Detection of Large Molecules in Matrix-assisted UV-laser Desorption.”Rapid Communication in Mass Spectrometry, vol. 4, #9, pp. 301-305 (1990).
M. Salehpour et al., “Laser-induced Desorption of Proteins.”Rapid Communications in Mass Spectrometry, vol. 3, #8, pp. 259-263 (1989).
M. Karas et al., “UV Laser Matrix Desorption/Ionization Mass Spectrometry of Proteins in the 100,000 Dalton Range.”International Journal of Mass Spectrometry and Ion Processes, vol. 92, pp. 231-242 (1989).
R.C. Beavis et al., “Cinnamic Acid Derivatives as Matrices for Ultraviolet Laser Desorption Mass Spectrometry of Proteins.”Rapid Communications in Mass Spectrometry, vol. 3, #12 pp. 432-435 (1989).
F. Hillenkamp et al., “Laser Desorption Ionization of Proteins with Molecular Masses Exceeding 10,000 Daltons.”Anal. Chem., vol. 60, pp. 2299-2301 (1988).
E.C. Apel et al., “Multiphoton Photoionization Mass Spectra of Nitrobenzene and 2,4,6-Trinitrotoluene.”International Journal of Mass Spectrometry and Ion Processes, vol. 70, pp. 243-246 (1986).
J. Smalley, “The Remote Measurement of Sulphur in Steels by Mass Spectrometry.”Vacuum, vol. 27, (#9), pp. 555-558 (Feb. 9, 1977).
Bai Jian
Fischer Steven M.
Flanagan J. Michael
Agilent Technologie,s Inc.
Gurzo Paul M.
Lee John R.
Orrick Herrington & Sutcliffe
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