Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2005-01-18
2005-01-18
Kunz, Gary (Department: 1647)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
C435S325000, C530S350000
Reexamination Certificate
active
06844148
ABSTRACT:
The present invention provides the enzyme and enzymatic procedures for cleaving the β secretase cleavage site of the APP protein and associated nucleic acids, peptides, vectors, cells and cell isolates and assays. An enzyme that cleaves the α-secretase site of APP also is provided. The invention further provides a modified APP protein and associated nucleic acids, peptides, vectors, cells, and cell isolates, and assays that are particularly useful for identifying candidate therapeutics for treatment or prevention of Alzheimer's disease.
REFERENCES:
patent: 5424205 (1995-06-01), Dovey et al.
patent: 5455169 (1995-10-01), Mullan
patent: 5593846 (1997-01-01), Schenk et al.
patent: 5733768 (1998-03-01), Dixon et al.
patent: 5744346 (1998-04-01), Chrysler et al.
patent: 5750349 (1998-05-01), Suzuki et al.
patent: 5766846 (1998-06-01), Schlossmacher et al.
patent: 5795963 (1998-08-01), Mullan
patent: 5837672 (1998-11-01), Schenk et al.
patent: 5849560 (1998-12-01), Abraham
patent: 5877015 (1999-03-01), Hardy et al.
patent: 5942400 (1999-08-01), Anderson et al.
patent: 6025180 (2000-02-01), Powell et al.
patent: 6162630 (2000-12-01), Powell et al.
patent: 6211428 (2001-04-01), Singh et al.
patent: 6221645 (2001-04-01), Chryster et al.
patent: 6245884 (2001-06-01), Hook
patent: 6245964 (2001-06-01), McLonlogue et al.
patent: 6291223 (2001-09-01), Christie et al.
patent: 6313268 (2001-11-01), Hook
patent: 6319689 (2001-11-01), Powell et al.
patent: 6358725 (2002-03-01), Christie et al.
patent: 6361975 (2002-03-01), Christie et al.
patent: 6545127 (2003-04-01), Tang et al.
patent: 6713276 (2004-03-01), Cordell et al.
patent: 0848 062 (1998-06-01), None
patent: 0855 444 (1999-07-01), None
patent: WO 9631122 (1996-10-01), None
patent: WO 9640885 (1996-12-01), None
patent: WO 9813488 (1998-04-01), None
patent: WO 9821589 (1998-05-01), None
patent: WO 9826059 (1998-06-01), None
patent: WO 9931236 (1999-06-01), None
patent: WO 9934004 (1999-08-01), None
patent: WO 9946281 (1999-09-01), None
patent: WO 9964587 (1999-12-01), None
patent: WO 0023576 (2000-04-01), None
patent: WO 0047618 (2000-08-01), None
patent: WO 0056871 (2000-09-01), None
patent: WO 0058479 (2000-10-01), None
patent: WO 0068266 (2000-11-01), None
patent: WO 0069262 (2000-11-01), None
patent: WO 0100663 (2001-01-01), None
patent: WO 0100665 (2001-01-01), None
patent: WO 0129563 (2001-04-01), None
patent: WO 0131054 (2001-05-01), None
patent: WO 0136600 (2001-05-01), None
patent: WO 0138487 (2001-05-01), None
Bodovitz et al., J. of Neruochemistry, 64(1):307-315, 1995.*
ATCC catalog No. CRL-1573.available from http://www.atcc.org/SearchCatalogs/Search.cfm, obtained May 5, 2003.*
Chyung et al., J. of Cell Biology, Aug. 11, 1997, 138(3):671-80.*
And Marx et al., Experimental Gerontol., 34(6):783-795, Sep. 1, 1999.*
U.S. Provisional application No. 60/141,363, Lin et al.
U.S. Provisional application No. 60/168,060, Lin et al.
U.S. Provisional application No. 60/178,368, Lin et al.
U.S. Provisional application No. 60/210,292, Hong et al.
U.S. patent application Ser. No. 09/277,229, Citron et al.
U.S. Provisional application No. 60/177,836, Lin et al.
U.S. Provisional application No. 60/119,571, Basi et al.
U.S. Provisional application No. 60/139,172, Anderson et al.
U.S. Provisional application No. 60/114,408, Basi et al.
U.S. patent application Ser. No. 09/404,578, Chrysler et al.
U.S. patent application Ser. No. 09/054,334, Anderson et al.
U.S. patent application Ser. No. 09/730,329, Anderson et al.
U.S. patent application Ser. No. 09/471,669, Anderson et al.
U.S. patent application Ser. No. 09/501,708, Anderson et al.
U.S. patent application Ser. No. 09/723,722, Anderson et al.
U.S. patent application Ser. No. 09/724,566, Anderson et al.
U.S. patent application Ser. No. 09/723,739, Anderson et al.
U.S. patent application Ser. No. 09/724,571, Anderson et al.
U.S. patent application Ser. No. 09/724,568, Anderson et al.
U.S. patent application Ser. No. 09/724,569, Anderson et al.
Chyung et al. Novel β-Secretase Cleavage of β-Amyloid Precursor Protein in the Endoplasmic Reticulum/Intermediate Compartment of NT2N Cells,Journal of Cell Biology,138: 671-680 (Aug. 11, 1997).
Evin et al., Alzheimer's disease amyloid precursor protein (AβPP): proteolytic processing, secretases and βA4 amyloid production,Amyloid; Int. J. Exp. Clin. Invest.1: 263-280 (Sep. 8, 1994).
Haass et al., Amyloid β-peptide is Produced by Cultured Cells During Normal Metabolism,Nature,359: 322-325 (Sep. 24, 1992).
Haass et al., β-Amyloid Peptide and 3-kDa Fragment are Derived by Distinct Cellular Mechanisms,Journal of Biochemistry,268: 3021-3024 (Feb. 15, 1993).
Haass et al., The Swedish Mutation Causes Early-Onset Alzheimer's Disease by β-Secretase Cleavage Within the Secretory Pathway,Nature Medicine,12: 1291-1296 (Dec. 1995).
Hirosawa et al., Characterization of cDNA Clones Selected by the GeneMark Analysis from Size-Fractionated cDNA Libraries From Human Brain,DNA Res.,6(5): 329-336 (Oct. 29, 1999).
Hussain et al., Identification of a Novel Aspartic Protease (Asp 2) as β-Secretase,Molecular and Cellular Neuroscience,14: 419-427 (1999).
Kang et al., The Precursor of Alzheimer's Disease Amyloid A4 Protein Resembles a Cell-Surface Receptor,Nature,325: 733-736 (Feb. 19, 1987).
Kitaguchi et al., Novel Precursor of Alzheimer's Disease Amyloid Protein Shows Protease Inhibitory Activity,Nature,331: 530-532 (Feb. 11, 1988).
Knops et al., Cell-type and Amyloid Precursor Protein-type Specific Inhibition of Aβ Release by Bafilomycin A1, a Selective Inhibitor of Vacuolar ATPases,Journal of Biological Chemistry,270: 2419-2422 (Feb. 10, 1995).
Koo and Squazzo Evidence that Production and Release of Amyloid β-Protein Involves the Endocytic Pathway,Journal of Biological Chemistry,269: 17386-17389 (Jul. 1, 1994).
Ponte et al., A New A4 Amyloid mRNA Contains a Domain Homologous to Serine Proteinase Inhibitors,Nature,331: 525-527 (Feb. 11, 1988).
Seubert et al. Secretion of β-amyloid Precursor Protein Cleaved at the Amino Terminus of the β-amyloid Peptide,Nature,361: 260-263 (Jan. 21, 1993).
Sinha et al., Purification and Cloning of Amyloid Precursor Protein β-Secretase from Human Brain,Nature,402: 537-540 (Dec. 2, 1999).
Szecsi, The Aspartic Proteases,Scand. J. Clin. Lab. Invest.,52 (suppl. 210): 5-22 (1992).
Tanzi et al., Protease Inhibitor Domain Encoded by an Amyloid Protein Precursor mRNA Associated with Alzheimer's Disease,Nature,331: 528-530 (Feb. 11, 1988).
Vasser et al., β-secretase Cleavage of Alzheimer's Amyloid Precursor Protein by the Transmembrane Aspartic Protease BACE,Science,286 (5440): 735-41 (Oct. 22, 1999).
Yan et al., Membrane-anchored Aspartyl Protease with Alzheimer's Disease β-Secretase Activity,Nature,402: 533-537 (Dec. 2, 1999).
Zhao et al., β-Secretase Processing of the β-Amyloid Precursor Protein in Transgenic Mice Is Efficient in Neurons but Inefficient in Astrocytes,Journal of Biological Chemistry,271: 31407-31411 (Dec. 6, 1996).
PCT Search report for PCT/US 99/20881.
Mullan et al., A Pathogenic Mutation for Probable Alzheimer's Disease in the APP Gene at the N-Terminus of β-Amyloid,Nature Genetics1: 345-347, (Aug. 1992).
Elan and Pharmacia form Alzheimer's disease research collaboration in the area of Beta-Secretase, News Aug. 9, 2000, www.elancorp.com.
Bienkowski Michael Jerome
Gurney Mark
Kunz Gary
Nichols Christopher James
Pharmacia & Upjohn Company
Pharmacia & Upjohn Company
Wootton Thomas A.
LandOfFree
Alzheimer's disease secretase, APP substrates therefor, and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Alzheimer's disease secretase, APP substrates therefor, and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Alzheimer's disease secretase, APP substrates therefor, and... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3378281