Alternative dye-labeled primers for automated DNA sequencing

Details Alternative dye-labeled primers for automated DNA sequencing Alternative dye-labeled primers for automated DNA sequencing

1995-10-06

1999-01-19

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 5, 435 912, 536 243, 536 2432, 536 2433, 536 231, C12P 1934, C12Q 168, C07H 2104, C07H 2102

Patent

active

058612874

ABSTRACT:
Methods for the use of a class of dyes for improved DNA sequencing are provided. A new class of dyes, BODIPY.RTM. fluorophores, has been described recently. The parent heterocyclic molecule of the BODIPY.RTM. fluorophores is a dipyrrometheneboron difluoride compound which is modified to create a broad class of spectrally-discriminating fluorophores. The present invention provides methods for the use of BODIPY.RTM. fluorophore-labeled DNA for dye-primer sequencing in which the BODIPY.RTM.s are attached to the 5' end of sequencing primers. BODIPY.RTM. fluorophores have improved spectral characteristics compared to conventional fluorescein and rhodamine dyes. BODIPY.RTM. fluorophores have narrower band width, insensitivity to solvent or pH, and improved photostability, thus, BODIPY.RTM. fluorophores lead to improved DNA sequencing and/or detection in any method where electrophoresis and detection of DNA is required. Additionally, the spectral properties of the BODIPY.RTM. fluorophores are sufficiently similar in wavelength and intensity to be used with conventional equipment known in the art.

REFERENCES:
patent: 4318846 (1982-03-01), Khanna
patent: 4744339 (1988-05-01), Haugland et al.
patent: 4755458 (1988-07-01), Rabbani
patent: 4774339 (1988-09-01), Haughland et al.
patent: 4811218 (1989-03-01), Hunkapiller et al.
patent: 4855225 (1989-08-01), Fung et al.
patent: 5151507 (1992-09-01), Hobbs
patent: 5171534 (1992-12-01), Smith et al.
patent: 5188934 (1993-02-01), Menchen et al.
patent: 5241060 (1993-08-01), Englehardt
patent: 5366603 (1994-11-01), Middendorf et al.
patent: 5366860 (1994-11-01), Bergot et al.
patent: 5370777 (1994-12-01), Guttman et al.
Metzker et al., Termination of DNA synthesis by novel 3'-modified-deoxyribonucleoside 5'-triphosphates, Nucleic Acids Research, 22:4259-4267 (1994).
Drmanac, et al., Sequencing of Megabase Plus DNA by Hybridization: Theory of the Method: Genomics, 4:114-28 (1989).
Khrapko et al., An oligonucleotide hybridization approach to DNA sequencing, FEB, 256:118-22 (1989).
Strezoska et al., DNA sequencing by hybridization: 100 bases read by a non-gel-based method; PNAS, 88:10089-93 (1991).
Boude et al., Enhanced DNA sequencing by hybridization; PNAS, 91:3072-76 (1994).
Pease et al., Light-generated oligonucleotide arrays for rapid DNA sequence analysis; PNAS, 91:5022-26 (1994).
Studier, A strategy for high-volume sequencing of cosmid DNAs: Random and directed priming with a library of oligonucleotides; PNAS, 86:6917-21 (1989).
Kieleczawa et al., DNA Sequencing by Primer Walking with Strings of Contiguous Hexamers; Science, 258:1787-91 (1992).
Swerdlow et al., Capillary gel electrophoresis for rapid, high resolution DNA sequencing; Nucleic Acids Research, 18:1415-19 (1990).
Karger et al., Multiwavelength fluorescence detection for DNA sequencing using capillary electrophoresis, Nucleic Acids Research, 19:4955-62 (1991).
Ruiz-Martinez et al., DNA Sequencing by Capillary Electrophoresis with Replaceable Linear Polyacrylamide and Laser-Inducted Fluorescence Detection; Anal Chem., 65:2851-58.
Prober et al., A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides, Science, 238:336-41 (1987).
Smith et al., Fluorescence detection in automated DNA sequence analysis; Nature, 321:674-79 (1986).
Lee et al., NAR 20: 247124-83, 1992.
Haugland, Report from Energy Research Abstracts 17(6) Abstr. No. 16972, 1990.

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