Altered gene and E. coli strains and methods useful in enhanced

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor


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4352528, 435 691, 536 2351, C12N 1531, C12N 121, C12N 1567, C12P 2100




An rpoH gene that encodes a .sigma..sup.32 protein in which cysteine instead of arginine is at amino acid residue 268 was isolated from a mutant of Escherichia coli strain W3110. The rpoH gene has thymine instead of cytosine at the nucleotide position corresponding to 802 in the wild-type rpoH gene. Purified strains of E. coli W3110 and JM101 having a mutant rpoH gene in which thymine instead of cytosine is at the nucleotide position corresponding to 802 in a wild-type rpoH gene and a method for synthesis of proteins at enhanced levels are also disclosed. The method comprises introducing an expression vehicle into an E. coli strain containing the mutant rpoH gene of this invention.

Obukowicz et al. (May 1992), Appl. Environ. Microbiol. 58(5): 1511-1523.
Grossman, Alan D. et al., "Mutations in the rpoH (hptR) gene of Escherichia coli K-12 phenotypically suppress a temperature-sensitive mutant defective in the sigma-70 subunit of RNA polymerase", J. Bacteriology 161:939-943 (1985).
Calendar, Richard et al., "Deletion and insertion mutations in the rpoH Gene of Escherichia coli that produce functional sigma-32", J. Bacteriology 170:3479-3484 (1988).
Obukowicz, Mark G. et al., "Secretion and export of IGF-1 in Escherichia coli strain JM101", Mol. Gen. Genet. 215:19-25 (1988).
Silhavy, Thomas J. et al., Experiments with Gene Fusions, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1984).
Grossman, Alan D. et al., "Mutations in the lon gene of E. coli K12 phenotypically suppress a mutation in the sigma subunit of RNA polymerase," Cell 32:151-159 (1983).
Gierse, James K. et al., "Expression, purification, and in viro activity of atrial natriuretic factor prohormone produced in Escherichia coli,:" Archiv. Biochem. Biophys., 271:441-446 (1989).
Wong, Edith Y. et al., "Expression of secreted insulin-like growth factor-1 in Escherichia coli," Gene 68:193-203 (1988).
Easton, Alan M. et al., "Production of bovine insulin-like growth factor-2 (bIGF-2) in Escherichia coli," Gene 101:291-295 (1990).
Schuler, Linda A. et al., "Bovine placental lactogen:molecular cloning and protein structure,"Biochemistry 27:8443-8448 (1988).
Miller, W. L. et al., "Cloning of bovine prolactin cDNA and evolutionary implications of its sequence," DNA 1:37-50 (1981).
Grossman, Alan D. et al., "The hptR gene product of E. coli is a sigma factor for heat-shock promoters," Cell, vol. 38, 383-390, Sep. 1984.
Grossman, A. D. et al., "Analysis of the Escherichia coli heat shock response," Microbiology-1985, Loretta Leive (ed.), American Society for Microbiology, Washington, D.C. (1985).
Grossman, Alan D. et al., ".sigma..sup.32 synthesis can regulate the synthesis of heat shock proteins in Escherichia coli", Genes & Development, 1:179-184, 1987.
Helmann, John D. and Chamberlin, Michael J., "Structure and Function of Bacterial Sigma Factors", Ann. Rev. Biochem., 1988, 57:839-72.
Straus, David et al., "DnaK, DnaJ, and GrpE heat shock proteins negatively regulate heat shock gene expression by controlling the synthesis and stability of .sigma..sup.32 ", Genes & Development, 4:2202-2209, 1990.
Yura, Takashi and Osawa, Toshio, "Genetics Studies and Evolution of RNA Polymerase, Sigma Factor in Escherichia coli", pp. 51-63, Genetics and Evolution of RNA Polymerase, trNA and Ribosomes, Osawa, S. et al., (ed.) Univ. of Tokyo Press, Tokyo, Japan.
Hu, James C. and Gross, Carol A., "Marker Rescue with Plasmids Bearing Deletions in RpoD Identifies a dispensible part of E. coli sigma factor", Mol. Gen. Genet. 1983, 191:492-298.
Bachmann, Barbara J., "Derivations and Genotypes of Some Mutant Derivatives of Escherichia coli K-12", pp. 1190-1219, Escherichia coli and Salmonella typhimurium, Neidhardt, F. C. et al. (ed.), American Society for Microbiology, Washington, D.C. (1987).
Sambrook, J. et al., "Molecular Cloning", pp. A.1, A.3, Cold Spring Harbor Laboratory Press, 1989.
Messing, J., "A Multipurpose Cloning system based on the single-stranded DNA Bacteriophage M13", Recombinant DNA Technical Bulletin, NIH Publication #79-99, vol. 2, No. 2, (1979) pp. 43-48.
Maloy, Stanley R. and Nunn, William D., "Selection of Loss of Tetracycline Resistance by Escherichia coli, Journal of Bacteriology", Feb. 1981, pp. 1110-1112.
Yano, Ryoji et al., "A Mutation that Enhances Synthesis of .sigma..sup.32 and Suppresses Temperature-Sensitive Growth of the rpoH15 Mutant of Escherichia coli", Journal of Bacteriology, Apr. 1990, pp. 2124-2130.
Tobe, Toru et al., "Suppression of rpoH (hptR) Mutations of Escherichia coli: Heat Shock Response in suhA Revertants", Journal of Bacteriology, Sep. 1987, pp. 4128-4134.
Tobe, Toru et al., "Isolation and Physical Mapping of Temperature-Sensitive Mutants Defective in Heat-Shock Induction of Proteins in Escherichia coli", Mol. Gen. Genet. (1984) 195:10-16.
Yura, Takashi et al., "Heat Shock Regulatory Gene (hptR) of Escherichia coli is Required for Growth at High Temperature But is Dispensable at Low Temperature", Proc. Natl. Acad. Sci. USA, vol. 81, pp. 6803-6807, Nov. 1984.
Bukau, Bernd et al., "Mutations Altering Heat Shock Specific Subunit of RNA Polymerase Suppress Major Cellular Defects of E. coli Mutants Lacking the DnaK Chaperone", The EMBO Journal, vol. 9, No. 12, pp. 4027-4038, 1990.
Baker, Tania A. et al., "A Gene Regulating the Heat Shock Response in Escherichia coli Also Affects Proteolysis", Proc. Natl. Acad. Sci. USA, vol. 81, pp. 6779-6783, Nov. 1984.
Cooper, Stephen et al., "A Temperature Sensitive Nonsense Mutation Affecting the Synthesis of a Major Protein of Escherichia coli K12", Molec. Gen. Genet. 139, 167-176 (1975).


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