Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide encodes an inhibitory rna molecule
Reexamination Certificate
2011-02-08
2011-02-08
Mehta, Ashwin (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide encodes an inhibitory rna molecule
C800S287000
Reexamination Certificate
active
07884263
ABSTRACT:
Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) inNicotianaplants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.
REFERENCES:
patent: 5668295 (1997-09-01), Wahab et al.
patent: 5684241 (1997-11-01), Nakatani et al.
patent: 2004/0103449 (2004-05-01), Xu
patent: 2004/0111759 (2004-06-01), Xu
patent: 2004/0117869 (2004-06-01), Xu
patent: 2004/0162420 (2004-08-01), Xu
patent: 2005/0132444 (2005-06-01), Xu
patent: 2006/0037096 (2006-02-01), Xu
patent: WO 00/67558 (2000-11-01), None
patent: WO 03/078577 (2003-09-01), None
patent: WO 2004/035745 (2004-04-01), None
patent: WO 2005/038018 (2005-04-01), None
patent: WO 2005/038033 (2005-04-01), None
patent: WO 2005/111217 (2005-11-01), None
patent: WO 2005/116199 (2005-12-01), None
patent: WO 2006/091194 (2006-08-01), None
Klahre et al., PNAS, 2002, vol. 99, pp. 11981-11986.
Gavilano et al., “Genetic Engineering ofNicotiana tabacumfor Reduced Nornicotine Content,” J.Agric. Food. Chem., 2006, pp. 9071-9078, vol. 54, No. 11.
Siminszky et al., “Conversion of Nicotine to Nornicotine inNicotiana tabacumIs Mediated by CYP82E4, a Cytochrome P450 Monooxygenase,”Proceedings of the National Academy of Sciences of USA, 2005, pp. 14919-14924, vol. 102, No. 41.
Tang et al., “Using RNAi to Improve Plant Nutritional Value: From Mechanism to Application,”Trends in Biotechnology, 2004, pp. 463-469, vol. 22, No. 9.
Chintapakorn, Y., et al., “Antisense-Mediated Down-Regulation of Putrescine N-Methyltransferase Activity in TransgenicNicotiana tabacumL. can Lead to Elevated Levels of Anatabine at the Expense of Nicotine,”Plant Molecular Biology, 2003, pp. 87-105, vol. 53.
Bowen Steven W.
Dewey Ralph E.
Gavilano Lily
Siminszky Balazs
Alston & Bird LLP
Mehta Ashwin
North Carolina State University
University of Kentucky Research Foundation
LandOfFree
Alteration of tobacco alkaloid content through modification... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Alteration of tobacco alkaloid content through modification..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Alteration of tobacco alkaloid content through modification... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2640898