ALS3 promoter

Details ALS3 promoter ALS3 promoter

1995-03-24

1997-08-19

Fox, David T.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 236, 435 691, 435 701, 4351723, 4353201, 435418, 435419, 800205, C12N 1529, C12N 504, C12N 1582, A01H 500

Patent

active

056590261

ABSTRACT:
The Brassica ALS3 promoter is operably linked to a foreign structural gene to provide high level, and generally constitutive or tissue general expression of the structural gene in transformed plants. The Brassica ALS3 promoter exhibits a non-tissue-preferred mode of expression at a level comparable to, and in some cases higher than, the widely used CaMV 35S promoter. Accordingly, DNA constructs comprising the Brassica ALS3 promoter operably linked to any number of different gene coding regions can be used for constitutive and tissue-general expression of the gene in transformed plants. The Brassica ALS3 promoter is used to direct expression of agronomically important genes and selectable marker genes.

REFERENCES:
Rutledge, et al. "Molecular characterization and genetic origin of the Brassica napus acetohydroxyacid synthase multigene family", Mol Gen Genet, 229:31-40 (1991).
Ouellet, et al. "Members of the acetohydroxyacid synthase multigene family of Brassica napus have divergent patterns of expression", The Planet Journal, 2(3):321-330 (1992).
Gabard, et al. "Cross-Resistance to Short Residual Sulfonylurea Herbicides in Transgenic Tobacco Plants", Plant Physiol., 91:574-580 (1989).
Wiersma, et al. "Isolation, expression and phylogenetic inheritance of an acetolactate synthase gene from Brassica napus", Mol Gen Genet, 219:413-420 (1989).
Miki, et al. "Transformation of Brassica napus canola cultivars with Arabidopsis thaliana acetohydroxyacid synthase genes and analysis of herbicide resistance", Theor Appl Genet, 80:449-458 (1990).
Hattori, et al. "An acetohydroxy acid synthase mutant reveals a single site involved in multiple herbicide resistance", Mol. Gen Genet, 246:419-425 (1995).
Brandle et al. A Comparison of Two Genes for Sulfonylurea Herbicide Resistance in Transgenic Tobacco Seedlings, Crop Science 34:226-229 (1994).
Tourneur, et al. Plant Science, vol. 88, pp. 159-168, 1993 "Over-expression of acetolactate synthase confers resistance to valine in transgenic tobacco".
Barbour, et al. Proceedings of the 1993 Annual Meeting of the CSPMB, p. 43, 1993 "Characterization of a B. Napus ALSIII gene and expression in transgenic plants".
Rutledge, et al. Mol. Gene Genet., vol. 229, pp. 31-40, 1991 "Molecular characterization and genetic origin of the Brassica napus acetohydroxyacid synthase multigene family".
Odell, et al. Plant Physiol., vol. 94, pp. 1647-1654, 1990 "Comparison of increased expression of wild-type and herbicide-resistant acetolactate synthase genes in transgenic plants, and indication of posttranscriptional limitation on enzyme activity".
Wiersma, et al. Mol. Gen Genetics, vol. 219, pp. 413-420, 1989 "Isolation, expression and phylogenetic inheritance of an acetolactate synthase gene from Brassica napus".
Barbour, et al. J. Cell Biochem. Suppl., p. 204, 1992 Abstract "Isolation and characterization of B Napus mutant ALS gene and expression in transgenic plants".

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