Alphavirus RNA replicon systems

Details Alphavirus RNA replicon systems Alphavirus RNA replicon systems

1998-07-24

2000-12-05

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

4353201, 435236, C12N 700, C12N 701, C12N 704

Patent

active

06156558&

ABSTRACT:
The present invention provides a helper cell for expressing an infectious, replication defective, alphavirus particle in an alphavirus-permissive cell. The helper cell includes (a) a first helper RNA encoding (i) at least one alphavirus structural protein, and (ii) not encoding at least one alphavirus structural protein; and (b) a second helper RNA separate from the first helper RNA, the second helper RNA (i) not encoding the alphavirus structural protein encoded by the first helper RNA, and (ii) encoding the at least alphavirus one structural protein not encoded by the first helper RNA, such that all of the alphavirus structural proteins assemble together into alphavirus particles in the cell. Preferably, the helper cell also includes a replicon RNA encoding an alphavirus packaging sequence and an inserted heterogeneous RNA.

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