.alpha.-glycosyl trehalose-forming enzyme

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435 95, 435 96, 435 97, 435 99, 435200, C12N 926, C12N 924, C12N 914, C12N 910

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active

054551684

ABSTRACT:
Disclosed is a novel non-reducing saccharide-forming enzyme. The enzyme can be obtained from the culture of microorganisms such as Rhizobium sp. M-11 (FERM BP 4130) and Arthrobacter sp. Q36 (FERM BP-4316), and is capable of forming non-reducing saccharides having a trehalose structure when allowed to act on reducing partial starch hydrolysates having a degree of polymerization of 3 or greater. Glucoamylase and .alpha.-glucosidase readily yield trehalose when allowed to act on the non-reducing saccharides. These non-reducing saccharides and trehalose are useful in food products, cosmetics and pharmaceuticals.

REFERENCES:
patent: 4521252 (1985-06-01), Miyake et al.
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"Current Status of Starch Application Development and Related Problems," Food Chemicals, No. 88, pp. 67-72, Aug. 1992.
Hoelzle et al., "Increased Accumulation of Trehalose in Rhizobia Cultured under 1% Oxygen," Applied and Environmental Microbiology, pp. 3213-3215, Oct. 1990.
Handbook of Amylases and Related Enzymes, The Amylase Research Society of Japan, Pergamon Press, 1988.
Bergey's Manual of Systematic Bacteriology, vol. 1, William & Wilkins, 1984.
Bergey's Manual of Systematic Bacteriology, vol. 2, William & Wilkins, 1986.
Advances in Carbohydrate Chemistry, Academic Press, 1963.
Journal of Clinical Nutrition, vol. 41, No. 2, pp. 200-208, (1972).
Journal of the Chemical Society, May 1965 Letchworth, GB, pp. 3489-3490, Birch, "A method of obtaining crystalline anhydrous alphaalpha-trehalose".
ATCC Catalogue of Bacteria and Bacteriophages, 17th Ed. 1989, pp. 46, 131, 141.
Lama et al, Biotech Letters 12(6): 431-432 (1990).

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