Alpha-complementation viral fusion assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

Reexamination Certificate

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C435S007210

Reexamination Certificate

active

10729069

ABSTRACT:
The present invention relates to the use of an alpha complementation assay as a quick, effective, and safe method for the detection of cell fusion mediated by viral proteins. Additionally, the method disclosed herein permits the identification of inhibitors of cell fusion using an alpha complementation assay.

REFERENCES:
patent: 5444161 (1995-08-01), Manning et al.
patent: 6096917 (2000-08-01), Hornback et al.
patent: 6100426 (2000-08-01), Mauldin et al.
patent: 6103922 (2000-08-01), Hornback et al.
patent: 6103923 (2000-08-01), Mauldin et al.
patent: 6124494 (2000-09-01), Mauldin et al.
patent: 6127422 (2000-10-01), Colacino et al.
patent: 6156924 (2000-12-01), Hornback et al.
patent: 6175034 (2001-01-01), Mauldin et al.
patent: 6180815 (2001-01-01), Mauldin et al.
patent: 6180816 (2001-01-01), Mauldin et al.
Bieniasz et al. HIV-1 induced cell fusion is mediated by multiple regions within both the viral envelope and the CCR-5 co-receptor 1997. Th EMBO Journal vol. 16, No. 10, pp. 2599-2609.
Wigley et al. Protein solubility and folding monitored in vivo by structural complementation of a genetic marker protein 2001. Nature Biotechnology vol. 19, pp. 131-136.
Isaacs et al. A macrophage fusion assay for rapid screening of cloned HIV-1 Env using dual recombinant vaccinia viruses expressing distinct RNA polymerases 1999. Journal of Virological Methods vol. 81, pp. 55-61.
Mohler W. Gene expression and cell fusion analyzed by lacZ complementation in mammalian cells. Proceedings from National Academy of Science USA. Oct. 1996, vol. 93, pp. 12423-12427.
Moir S. Expression of HIV env gene in a human T cell line for a rapid and quantifiable cell fusion assay. AIDS Research and Human Retroviruses. 1996, vol. 12, No. 9, pp. 811-820.
Doranz et al. A Small-molecule Inhibitor Directed against the Chemokine Receptor CXCR4 Prevents its Use as an HIV-1 Coreceptor. J. Exp. Med. Oct. 20, 1997, vol. 186, No. 8, p. 1395-1400.
Allaway et al.,AIDS Res. Hum. Retrovir. 11:533-539 (1995).
Baba et al.,Proc. Nat'l. Acad. Sci. U.S.A. 96(10):5698-5703 (1999).
Chan et al.,Cell89:263-273 (1987).
Dalgleish et al.,Nature312:763-767 (1984).
Deng et al.,Nature381:661-666 (1996).
Donzella et al.,Nat. Med. 4:72-77 (1998).
Dutch et al.,Biosci. Rep. 20(6):597-612 (2000).
Graham et al.,Virol. 52:456-67 (1973).
Hammarskjold et al.,Biochem. Biophys. Acta. 989:269-280 (1989).
Hope et al.,Proc. Nat'l. Acad. Sci. U.S.A.87:7787-91 (1990).
Klatzmann et al.,Nature312:767-768 (1984) .
Langley and Zabin,Biochemistry15:4866 (1976).
Lenburg and Landau,J. Virol. 67:7238-45 (1993).
Light et al.,J. Biol. Chem. 249:2285 (1974).
Maddon et al.,Cell42:93-104 (1985).
Maddon et al.,Cell47:333-348 (1986).
McDougal et al.,Science231:382-385 (1986).
Moosmann and Rusconi,Nucleic Acids Res. 24(6):1171-1172 (1996).
Morgenstern and Land,Nucleic Acids Res. 18(12):3587-96 (1990).
Onishi et al.,Exp. Hematol. 24:324-329 (1996).
Overbaugh et al.,Microbiol.&Mol. Biol. Rev. 65:371-389 (2001).
Raviv et al.,Virology293:243-51. (2002).
Richards and Vithayathil,J. Biol. Chem. 234:1459-1465 (1959).
Söllner et al.,Nature362:318-24 (1993).
Strizki et al.,Proc. Nat'l. Acad. Sci. U.S.A.98(22):12718-12723 (2001).
Sutton et al.,Nature395:347-53 (1998).
Vodicka et al.,Virology233:193-98 (1997).
Wild et al.,Proc. Nat'l. Acad. Sci. U.S.A.91(21):9770-9774 (1994).
York-Higgins et al.,J. Virol. 64:4016-4020 (1990).
Moosmann and Rusconi, Nucleic Acids Research , 24(6):1171-1172 (1996).
Richard Horuk, et al., “The CC Chemokine I-309 Inhibits CCR8-dependent Infection by Diverse HIV-1 Strains”, The Journal of Biological Chemistry, Jan. 2, 1998, 273(1), pp. 386-391.

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