Alpha-amylase variants

Details Alpha-amylase variants Alpha-amylase variants

2007-01-16

2007-01-16

Slobodyansky, Elizabeth (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S022000, C435S252300, C435S440000, C536S023200, C702S019000

Reexamination Certificate

active

10926720

ABSTRACT:
The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprising i) analyzing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and iii) testing the resulting Termamyl-like alpha-amylase variant for the property in question.

REFERENCES:
Machius et al., (1995) J. Mol. Biol. 246:545-559.
MacGregor (1988) J. Protein Chemistry 7:399-415.
Suzuki et al., (1989) J. Biol. Chem. 264:18933-18938.

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