.alpha.-amylase mutants

Details .alpha.-amylase mutants .alpha.-amylase mutants

1996-02-13

1999-11-23

Wax, Robert A.

Gas: heating and illuminating

435202, 435203, 435204, 4352523, 485440, 536 232, C12N 926, C12N 928, C12N 1500, C07H 2104

Patent

active

059891696

ABSTRACT:
The present invention relates to a method of constructing a variant of a parent Termamyl-like .alpha.-amylase, which variant has .alpha.-amylase activity and at least one altered property as compared to the parent .alpha.-amylase, comprising i) analyzing the structure of the parent Termamyl-like .alpha.-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like .alpha.-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like .alpha.-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like .alpha.-amylase variant, which as compared to the parent Termamyl-like .alpha.-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and iii) testing the resulting Termamyl-like .alpha.-amylase variant for the property in question.

REFERENCES:
Machius et al. (Mar. 3, 1995) J. Mol. Biol. vol. 246, pp. 545-559.
MacGregor (1988) J. Protein Chemistry 7, 399-415.
Suzuki et al. (1989) J. Biol. Chem. 264, 18933-18938.

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