.alpha.-amylase inhibitor

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Plant proteins – e.g. – derived from legumes – algae or...

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435 22, 435 68, 514 2, C07K 302, C07K 1510, C12P 2100, C12Q 140

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active

048066265

ABSTRACT:
New .alpha.-amylase inhibitor from wheat (WAI-53). The inhibitor is characterized especially by having molecular weight of 24,000 as determined by gel filtration, giving a single band at an electrophoretic mobility of 0.53 on polyacrylamide gel electrophoresis according to the Davis Disc Electrophoresis Method and producing saturation curves of WAI-53 with human salivary alpha-amylase and with human pancreatic alpha-amylase with a ratio of the amount of WAI-53 required to produce 50% inhibition of human salivary .alpha.-amylase to that of human pancreatic amylase above 1:250. The alpha-amylase inhibitor is used for quantitative analysis of alpha-amylase isozymes in body fluid.

REFERENCES:
O'Donnell et al; Biochim. Biophys. ACTA 422: 159 (1976).
Silano et al, "Inhibition of Amylases from Different Origins by Albumins from the Wheat Kernel", Biochim. Biophys. Acta 391: 170 (1975).

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